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General Information
Symbol
Dmel\IKKεdsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0194832
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Nucleotides 1-500 of the IKKε cDNA has been inserted as an inverted repeat and driven by UAS sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4ppk.PG

microtubule & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4ppk.PG

Detailed Description
Statement
Reference

Bristles are shorter and thicker in the adult notum of Scer\GAL4sca-P309; ik2dsRNA.Scer\UAS flies.

The proximal dendrites remain intact for most ddaC neurons at 18 hours after puparium formation (APF) in animals expressing ik2dsRNA.Scer\UAS under the control of Scer\GAL4ppk.PG, indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals).

The microtubules remain intact in the dendrites of ddaC neurons at 6 hours after puparium formation (APF) in animals expressing ik2dsRNA.Scer\UAS under the control of Scer\GAL4ppk.PG (microtubules in the dendrites of wild-type ddaC neurons show breakage at this stage).

Expression of ik2dsRNA.Scer\UAS in S2 cells, under the control of Scer\GAL4Act5C.PI, does not affect cell viability. However, these S2 cells show an increased likelihood to have a serrate or stellate morphology, instead of the typical round morphology.

Embryos treated with ik2 RNAi, generated by expression of ik2dsRNA.Scer\UAS under the control of Scer\GAL4btl.PS, show tracheal terminal branching defects. These include the duplication of terminal cells and the misorientation of the actin core.

Flies treated with ik2 RNAi, generated by expression of ik2dsRNA.Scer\UAS under the control of Scer\GAL4ap-md544, show gnarled, forked macrochaetae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Overexpression of thScer\UAS.T:Zzzz\FLAG enhances the short-bristle phenotype of Scer\GAL4sca-P309; ik2dsRNA.Scer\UAS flies.

Co-expression of NcC318G.Scer\UAS.T:Avic\GFP-EGFP in Scer\GAL4sca-P309; ik2dsRNA.Scer\UAS flies significantly enhances the short-bristle phenotype.

Xenogenetic Interactions
Statement
Reference

Co-expression of BacA\p35Scer\UAS.cHa and ik2dsRNA.Scer\UAS, both under the control of Scer\GAL4sca-P309 generates a short bristle phenotype, as found in Scer\GAL4sca-P309; ik2dsRNA.Scer\UAS mutants.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid and transfected into S2 cells. Transcription from the plasmid should produce ik2 dsRNA, resulting in dsRNA interference (RNAi) of the ik2 gene.

Carried in plasmid "pUAST-DmIKKε IR" and transfected into S2 cells. Transcription from the P{UAS-ik2.IR} construct should produce ik2 dsRNA, resulting in dsRNA interference (RNAi) of the ik2 gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
IKKεdsRNA.Scer\UAS
IKKεdsRNA.UAS
ik2dsRNA.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (6)