FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sec620
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General Information
Symbol
Dmel\Sec620
Species
D. melanogaster
Name
FlyBase ID
FBal0195148
Feature type
allele
Associated gene
Dmel\Sec6
Associated Insertion(s)
Carried in Construct
Also Known As
Sec6Δ20
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Beronja et al., 2005)
Mutagen
P-element activity
(Beronja et al., 2005)
Progenitor genotype
CthEP2021
(Beronja et al., 2005)
Cytology
Description

sec620 is the product of an imprecise excision of P{EP}EP2021 that deletes the DNA encoding the 242 carboxy-terminal amino acids of the sec6 protein.

(Beronja et al., 2005)

sec20 does not affect the 3' neighbouring gene, Eip55E.

(Beronja et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Sec620/Sec620 class IV dendritic arborizing neurons in larval somatic clones show defects in dendritic growth: significant reduction in both total dendrite length and number of terminal dendrites.

      (Peng et al., 2015)

      sec620 mutant animals fail to grow as larvae and die at approximately 72 hours after egg laying.

      sec620 hemizygous and homozygous animals show identical phenotypes.

      Follicles containing a sec620 germ-line clone show no apparent defects during early stages. However, by stage 6-7, plasma membrane is undetectable between germ-line cells, ring canals collapse to a single clump within the germline cyst, and nuclei show an irregular arrangement. sec620 mutant follicles are not observed past stage 7, suggesting that they degenerate.

      Eye development is almost abolished when sec620 mutant clones are induced in the eye and wild type sister clones are ablated.

      Eyes that are mutant for sec620 (made by inducing sec620 mutant clones and genetically ablating wild type sister clones) and which also express sec6Scer\UAS.P\T.cBa under the control of Scer\GAL4ey.PH are referred to as 'sec6(pr)' (for 'partial rescue') eyes. sec6(pr) mutant eyes develop to near normal size in the adult but are slightly rough and interommatidial bristles are virtually absent. Defects in the differentiation of the cornea and the pigment cells are apparent that are more severe in the anterior half of the eye. At 55% pupal development, gross morphological defects are not apparent in sec6(pr) mutant photoreceptors, although there are some defects in the organization of microvilli and a conspicuous increase in cytoplasmic vesicles compared to wild type. At 90% pupal development, sec6(pr) mutant photoreceptors appear swollen and have smaller and flattened rhabdomeres compared to wild type, and their cytoplasm is filled with vesicles of 100-300 nm diameter that are only rarely seen in wild type; the proximal-distal length of sec6(pr) rhabdomeres is significantly reduced compared to wild type, and the sec6(pr) retina as a whole reaches only approximately half the depth of a wild type retina; some sec6(pr) photoreceptor cells also have an enlarged endoplasmic reticulum. In one-day-old adult flies, the photoreceptors and other retinal cells of sec6(pr) eyes show widespread signs of degeneration; the rhabdomeres of photoreceptors are either strongly reduced in size or are missing.

      (Beronja et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Sec620 is rescued by Sec6UAS.C-YFP/Scer\GAL45-40

      (Peng et al., 2015)
      Partially rescued by

      Sec620 is partially rescued by Sec6UASp.cBa/Scer\GAL4ey.PH

      (Beronja et al., 2005)
      Comments

      Expression of Sec6Scer\UAS.T:Avic\GFP-CYFP under the control of Scer\GAL45-40 in Sec620/Sec620 class IV dendritic arborizing neurons in larval somatic clones fully rescues the dendritic growth defects displayed by Sec620/Sec620 neurons (reduction in both total dendrite length and number of terminal dendrites).

      (Peng et al., 2015)

      Expression of sec6Scer\UAS.P\T.cBa under the control of Scer\GAL4ey.PH substantially rescues the 'small eye' phenotype seen when sec620 mutant clones are induced in the eye and wild type sister clones are ablated.

      (Beronja et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Isolated as an imprecise excision of P{EP}EP2021.

      (Beronja et al., 2005)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Sec620
      Sec6Δ20
      (Peng et al., 2015)
      sec620
      (Beronja et al., 2005)
      sec6Δ20
      (Calero-Cuenca et al., 2016)
      sec6Δ20
      (Venken, 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)