Knockdown of shrbdsRNA.Scer\UAS driven in the class IV dendritic arborizing sensory neuron ddaC by Scer\GAL4ppk.1.9 leads to significant disruptions in pruning at 18hr after puparium formation, with dendrites still attached to the cell body (resulting from a lack of branch severing and clearance defects). Knockdown of shrbdsRNA.Scer\UAS driven in the class IV dendritic arborizing sensory neuron ddaC by Scer\GAL4ppk.1.9 leads to accumulation of ubiquitinated proteins (defects in multivesicular body formation) in third instar larvae. Knockdown of shrbdsRNA.Scer\UAS driven in class I da sensory neurons by Scer\GAL4smid-C161 or in mushroom body gamma neurons by Scer\GAL4Tab2-201Y leads to significant disruption in dendrite or axon (respectively) severing at 24hr after puparium formation, compared to controls.
Downregulation of shrb, through expression of shrbdsRNA.Scer\UAS in ddaE and ddaF neurons in the dorsal cluster under the control of Scer\GAL4221, generates a phenotype that is similar to that in wild-type. However, the number of dendritic termini is increased significantly due to an increase in fine, higher-order branches.