The Sec15 genomic locus flanked by 2Kb on upstream sequence and 1Kb of downstream sequence, amplified with primers: 5'-AAAAAAGCGGCCGCcatgcacagggatatgttgc-3' and 5'-AAAGGATCCtccttctgcactggaaggag-3' (revision of data in FBrf0188181).
Position of rescue fragment on reference sequence inferred by FlyBase curator.
Sec15+t5 rescues Sec15unspecified
Second instar lethality due to sec15unspecified is rescued by sec15+t5.