FlyBase Allele Report: Dmel\Sec10[RNAi.UAS]

General Information

Symbol

Dmel\Sec10^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0197848

Feature type

allele

Associated gene

Dmel\Sec10

Associated Insertion(s)

Carried in Construct

P{UAS-Sec10.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Andrews et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Andrews et al., 2002)

Mutagen

in vitro construct

(Andrews et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Sec10.RNAi}

Cytology

Description

UASt sequences drive expression of a hairpin loop of Sec10 sequence consisting of sense and antisense sequence for exon2 separated by sense intron 2 sequence as a spacer.

(Andrews et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Sec10

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

abnormal developmental rate | second instar larval stage, with Scer\GAL4^αTub84B.PL

(Andrews et al., 2002)

lethal - all die before end of larval stage, with Scer\GAL4^Feb36

(Andrews et al., 2002)

lethal - all die before end of second instar larval stage, with Scer\GAL4^2-286

(Andrews et al., 2002)

lethal - all die before end of second instar larval stage, with Scer\GAL4^69B

(Andrews et al., 2002)

lethal - all die before end of second instar larval stage, with Scer\GAL4^c323a

(Andrews et al., 2002)

lethal - all die before end of second instar larval stage, with Scer\GAL4^e22c

(Andrews et al., 2002)

lethal - all die before end of second instar larval stage, with Scer\GAL4^αTub84B.PL

(Andrews et al., 2002)

some die during larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

some die during second instar larval stage, with Scer\GAL4^2-286

(Andrews et al., 2002)

some die during second instar larval stage, with Scer\GAL4^69B

(Andrews et al., 2002)

some die during second instar larval stage, with Scer\GAL4^c323a

(Andrews et al., 2002)

some die during second instar larval stage, with Scer\GAL4^e22c

(Andrews et al., 2002)

some die during second instar larval stage, with Scer\GAL4^αTub84B.PL

(Andrews et al., 2002)

some die during third instar larval stage, with Scer\GAL4^Feb36

(Andrews et al., 2002)

viable, with Scer\GAL4^4G

(Andrews et al., 2002)

viable, with Scer\GAL4^34B

(Andrews et al., 2002)

viable, with Scer\GAL4^48Y

(Andrews et al., 2002)

viable, with Scer\GAL4^386Y

(Andrews et al., 2002)

viable, with Scer\GAL4^c698a

(Andrews et al., 2002)

viable, with Scer\GAL4^elav-C155

(Andrews et al., 2002)

viable, with Scer\GAL4^I-76-D

(Andrews et al., 2002)

viable, with Scer\GAL4^{l(3)31-1-31-1}

(Andrews et al., 2002)

viable, with Scer\GAL4^Mhc.PW

(Andrews et al., 2002)

viable, with Scer\GAL4^tey-5053A

(Andrews et al., 2002)

viable, with Scer\GAL4^twi.PG

(Andrews et al., 2002)

viable, with Scer\GAL4^V49

(Andrews et al., 2002)

viable, with Scer\GAL4^V85

(Andrews et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Sec10^{dsRNA.Scer\UAS} driven by Scer\GAL4^phm.PO leads to developmental delay/arrest at the larval stage; knockdown also disrupts trafficking of transmembrane proteins to the plasma membrane.

(Yamanaka et al., 2015)

sec10^{dsRNA.Scer\UAS}; Scer\GAL4^αTub84B.PL animals die as second instar larvae, although some are arrested at this stage and live for up to five days after egg laying.

sec10^{dsRNA.Scer\UAS} animals with Scer\GAL4^elav-C155 or Scer\GAL4^Mhc.PW have normal numbers of boutons per synaptic terminal at neuromuscular junctions. Marker localisation at these synapses suggest that vesicles and neurotranasmitter receptors are localised as in wild-type. Excitatory Junction Current (EJC) recordings at these synapses also appear normal. miniEJC recordings were also largely normal except for a small but statistically significant increase in (p < 0.03) reduction in spontaneous vesicle fusion rate (1.09 ± 0.12 Hz compared to 2.12 ± 0.26 Hz in wild type) with Scer\GAL4^elav-C155.

Denticle belt structures are normal in sec10^{dsRNA.Scer\UAS}; Scer\GAL4^69B larvae. Polarised secretion across the midgut appears normal in sec10^{dsRNA.Scer\UAS}; Scer\GAL4^e22c larvae.

sec10^{dsRNA.Scer\UAS}; Scer\GAL4^Feb36 larvae die during the third instar prior to pupation. However, pupation in these larvae can be rescued by adding ecdysone (20E) to their food.

(Andrews et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Use to express double stranded RNA for the purposes of disrupting sec10 expression by RNAi.

(Andrews et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Sec10^RNAi.UAS

Sec10^{dsRNA.Scer\UAS}

Sec10^dsRNA.UAS

sec10^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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