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General Information
Symbol
Dmel\Sec10dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0197848
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of a hairpin loop of Sec10 sequence consisting of sense and antisense sequence for exon2 separated by sense intron 2 sequence as a spacer.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Sec10dsRNA.Scer\UAS driven by Scer\GAL4phm.PO leads to developmental delay/arrest at the larval stage; knockdown also disrupts trafficking of transmembrane proteins to the plasma membrane.

sec10dsRNA.Scer\UAS; Scer\GAL4αTub84B.PL animals die as second instar larvae, although some are arrested at this stage and live for up to five days after egg laying.

sec10dsRNA.Scer\UAS animals with Scer\GAL4elav-C155 or Scer\GAL4Mhc.PW have normal numbers of boutons per synaptic terminal at neuromuscular junctions. Marker localisation at these synapses suggest that vesicles and neurotranasmitter receptors are localised as in wild-type. Excitatory Junction Current (EJC) recordings at these synapses also appear normal. miniEJC recordings were also largely normal except for a small but statistically significant increase in (p < 0.03) reduction in spontaneous vesicle fusion rate (1.09 ± 0.12 Hz compared to 2.12 ± 0.26 Hz in wild type) with Scer\GAL4elav-C155.

Denticle belt structures are normal in sec10dsRNA.Scer\UAS; Scer\GAL469B larvae. Polarised secretion across the midgut appears normal in sec10dsRNA.Scer\UAS; Scer\GAL4e22c larvae.

sec10dsRNA.Scer\UAS; Scer\GAL4Feb36 larvae die during the third instar prior to pupation. However, pupation in these larvae can be rescued by adding ecdysone (20E) to their food.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Use to express double stranded RNA for the purposes of disrupting sec10 expression by RNAi.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Sec10dsRNA.Scer\UAS
Sec10dsRNA.UAS
sec10dsRNA.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)