Expression of Sec10dsRNA.Scer\UAS driven by Scer\GAL4phm.PO leads to developmental delay/arrest at the larval stage; knockdown also disrupts trafficking of transmembrane proteins to the plasma membrane.
sec10dsRNA.Scer\UAS; Scer\GAL4αTub84B.PL animals die as second instar larvae, although some are arrested at this stage and live for up to five days after egg laying.
sec10dsRNA.Scer\UAS animals with Scer\GAL4elav-C155 or Scer\GAL4Mhc.PW have normal numbers of boutons per synaptic terminal at neuromuscular junctions. Marker localisation at these synapses suggest that vesicles and neurotranasmitter receptors are localised as in wild-type. Excitatory Junction Current (EJC) recordings at these synapses also appear normal. miniEJC recordings were also largely normal except for a small but statistically significant increase in (p < 0.03) reduction in spontaneous vesicle fusion rate (1.09 ± 0.12 Hz compared to 2.12 ± 0.26 Hz in wild type) with Scer\GAL4elav-C155.
Denticle belt structures are normal in sec10dsRNA.Scer\UAS; Scer\GAL469B larvae. Polarised secretion across the midgut appears normal in sec10dsRNA.Scer\UAS; Scer\GAL4e22c larvae.
sec10dsRNA.Scer\UAS; Scer\GAL4Feb36 larvae die during the third instar prior to pupation. However, pupation in these larvae can be rescued by adding ecdysone (20E) to their food.