FlyBase Allele Report: Dmel\Vps25[Pb2931]

General Information

Symbol

Dmel\Vps25^Pb2931

Species

D. melanogaster

Name

FlyBase ID

FBal0197867

Feature type

allele

Associated gene

Dmel\Vps25

Associated Insertion(s)

PBac{PB}Vps25^Pb2931

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Thompson et al., 2005)

Progenitor genotype

Associated Insertion(s)

PBac{PB}Vps25^Pb2931

Cytology

Description

PBac{PB}Vps25^Pb2931 is located in the 5' UTR sequence of Vps25, five nucleotides upstream of the starting ATG codon.

(Thompson et al., 2005)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:8,625,521..8,625,521

(Thompson et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA

(Thompson et al., 2005)

CEA with BacA\p35^UAS.cHa

(Thompson et al., 2005)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | germline clone | recessive

(Irion and St Johnston, 2007)

hyperplasia | somatic clone

(Thompson et al., 2005)

increased cell death | cell autonomous | somatic clone

(Thompson et al., 2005)

lethal - all die before end of larval stage | recessive

(Thompson et al., 2005)

some die during larval stage | recessive

(Thompson et al., 2005)

Phenotype Manifest In

egg | germline clone

(Irion and St Johnston, 2007)

eye | somatic clone

(Thompson et al., 2005)

leg | somatic clone

(Thompson et al., 2005)

wing | somatic clone

(Thompson et al., 2005)

Detailed Description

Statement

Reference

Females with homozygous germline clones do not lay eggs.

(Irion and St Johnston, 2007)

Somatic clones of Vps25^Pb2931 homozygous cells form only tiny clones in the adult eye, but non-autonomously stimulate cell growth. Somatic clones in the proximal wing can induce patterned outgrowths, and clones in the leg can induce branching from the ventral side of the leg in which leg structures proximal to the outgrowth are duplicated. (Clone induction: 1 hour heat shock between 48 & 92 hours after egg laying).

The survival of Vps25^Pb2931 homozygous clones in imaginal discs is rescued if the clones also 'Minute'[+] and are in a 'Minute' heterozygous animal. The resulting clones exhibit massive overgrowth.

(Thompson et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Vps25^Pb2931 has increased cell death | somatic clone | cell autonomous phenotype, suppressible | somatic clone by Scer\GAL4^αTub84B.PL/BacA\p35^UAS.cHa

(Thompson et al., 2005)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^αTub84B.PL, Vps25^Pb2931 has hyperplasia | somatic clone phenotype

(Thompson et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The survival of Vps25^Pb2931 homozygous clones in imaginal discs is rescued if the clones are also carry BacA\p35^Scer\UAS.cHa and Scer\GAL4^αTub84B.PL but lack Scer\GAL80^αTub84B.PL and are in an animal which carries Scer\GAL80^αTub84B.PL. The resulting clones exhibit massive overgrowth and complete loss of epithelial integrity - the cells are rounded and adopt a loose mesenchymal arrangement. Surviving wild-type tissue also exhibits overgrowth, but keep its epithelial character - albeit distorted by overgrowth. In the eye, cells in these clones lack normal signs of neuronal differentiation. When these 'tumorous' discs are transplanted into the abdomen of an adult female, tumorous tissue was found in other parts of the animal in some cases (2/16) after 5 days. These metastases were not found in control females into which wild-type discs had been implanted.

(Thompson et al., 2005)

Complementation and Rescue Data

Fails to complement

Vps25^k08904

(Thompson et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The lethality of Vps25^Pb2931 homozygotes is rescued by precise excision of PBac{PB}Vps25^Pb2931.

(Thompson et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Vps25^Pb2931

(Thompson et al., 2005)

vps25^PB2931

(Grusche et al., 2011)

vps25^Pb2931

(Irion and St Johnston, 2007, Irion and St Johnston, 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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