Scer\GAL4^da.G32>4EHP^GD7071 larvae exhibit extreme developmental delay: 5 days after egg deposition, mutant larvae are significantly smaller than controls and show mouth hook morphology characteristic of second instar larvae - they die 3-4 days later.

Larvae with knockdown of 4EHP^GD7071 driven by Scer\GAL4^tub.PU specifically at the early third larval instar stage (using Scer\GAL80^ts.αTub84B put at non-permissive 29[o]C 5 days after egg deposition) do not develop into adulthood: most do not pupate and instead form abnormally large third instar larvae that survive 3-4 weeks before dying.

Scer\GAL4^phm.PO>4EHP^GD7071 third instar larvae are slightly smaller (not significant) than controls and do not pupate, instead continuing to feed and increasing in size (significantly larger than controls 19 days after egg deposition) during a prolonged third instar larval stage (3-4 weeks AED). Prothoracic glands (PG) are significantly smaller than controls in Scer\GAL4^phm.PO>4EHP^GD7071 larvae, with a significant reduction in PG cell area. Larval developmental arrest induced by knockdown of 4EHP^GD7071 driven by Scer\GAL4^phm.PO is rescued by sterol-feeding: third instar larvae fed ecdysone show partial rescue (50% pupate) and 20E feeding leads to full rescue (all pupate), whereas feeding of cholesterol or ethanol has no effect. Using Scer\GAL80^ts.αTub84B at non-permissive 29[o]C to control timing of Scer\GAL4^phm.PO>4EHP^GD7071 knockdown, larvae arrest development at the third instar stage when knockdown occurs at early second instar stage (L2) or earlier (larvae pupate normally if knockdown occurs at later stages); PG size with L2 knockdown is normal or larger than controls, suggesting PG size does not correlate with larval developmental arrest.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Adults expressing 4EHP^GD7071 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 40-50% or 70-80% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.