FlyBase Allele Report: Dmel\ALiX[GD7853]

General Information

Symbol

Dmel\ALiX^GD7853

Species

D. melanogaster

Name

FlyBase ID

FBal0198598

Feature type

allele

Associated gene

Dmel\ALiX

Associated Insertion(s)

Carried in Construct

P{GD7853}

Also Known As

UAS-ALiX-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7853}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7853

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Knockdown of ALiX^GD7853 driven in the class IV dendritic arborizing sensory neuron ddaC by Scer\GAL4^ppk.1.9 does not lead to pruning defects at 18hr after puparium formation.

(Loncle et al., 2015)

Intraluminal vesicles inside MVBs or lysosomes (MVBLs) are almost completely absent in male secondary cells expressing ALiX^GD7853 under the control of Scer\GAL4^spi-NP0261. Maturing MVBLs (mMVBLs) are smaller than controls. The numbers of immature late endosomes (iLEs) and mMVBLs per secondary cell are similar to controls. Exosome secretion into the accessory gland lumen is reduced compared to controls when ALiX^GD7853 is expressed under the control of Scer\GAL4^dsx.KI.

Expression of ALiX^GD7853 under the control of Scer\GAL4^esg-NP7397 reduces the ability of males to prevent female remating. Mating times are reduced, but there are no significant effects on egg laying or fecundity.

The nuclei of accessory gland secondary cells expressing ALiX^GD7853 under the control of Scer\GAL4^esg-NP7397 are reduced in size compared compared to neighbouring main cell nuclei.

(Corrigan et al., 2014)

Adults expressing ALiX^GD7853 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development