FlyBase Allele Report: Dmel\Actn[GD1354]

General Information

Symbol

Dmel\Actn^GD1354

Species

D. melanogaster

Name

FlyBase ID

FBal0198670

Feature type

allele

Associated gene

Dmel\Actn

Associated Insertion(s)

Carried in Construct

P{GD1354}

Also Known As

UAS-Actn-IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1354}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1354

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

muscle attachment site | larval stage, with Scer\GAL4^Mef2.PR

(Clark and Kadrmas, 2013)

nephrocyte diaphragm | larval stage, with Scer\GAL4^sns.PK

(Odenthal et al., 2023)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Expression of Actn^GD1354 under the control of Scer\GAL4^Mef2.PR causes larvae to develop more slowly than controls; newly-hatched (0-12 hour) larvae are smaller than controls, and, although their muscles have prominent Z-lines, some muscles appear to pull away from the attachment sites.

(Clark and Kadrmas, 2013)

Adults expressing Actn^GD1354 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Actn^GD1354 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or complete lethality, depending on the particular P{GD1354} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early pupal lethality or late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in split larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with reduced Z-lines in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 90-100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 10-20% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression of argos^GD1666 under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF leads to a mild rough eye phenotype.

(Dietzl et al., 2007)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1354

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Actn^GD1354, Scer\GAL4^Mef2.PR has some die during larval stage phenotype, enhanceable by Mlp84B^GD6893, Scer\GAL4^Mef2.PR

(Clark and Kadrmas, 2013)

Actn^GD1354, Scer\GAL4^Mef2.PR has decreased body size | larval stage phenotype, enhanceable by Mlp84B^GD6893, Scer\GAL4^Mef2.PR

(Clark and Kadrmas, 2013)

Actn^GD1354, Scer\GAL4^Mef2.PR has visible | larval stage phenotype, enhanceable by Mlp84B^GD6893, Scer\GAL4^Mef2.PR

(Clark and Kadrmas, 2013)

Actn^GD1354, Scer\GAL4^Mef2.PR has some die during larval stage phenotype, enhanceable by Mlp84B^PΔ8-95/Mlp84B[+]

(Clark and Kadrmas, 2013)

NOT Enhancer of

Statement

Reference

Actn^GD1354, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

NOT Suppressor of

Statement

Reference

Actn^GD1354, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Actn^GD1354, Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH has eye phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

NOT suppressed by

Statement

Reference

Actn^GD1354 has muscle attachment site | larval stage phenotype, non-suppressible by Mlp84B^GD6893/Scer\GAL4^Mef2.PR

(Clark and Kadrmas, 2013)

NOT Enhancer of

Statement

Reference

Actn^GD1354, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

NOT Suppressor of

Statement

Reference

Actn^GD1354, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

Other

Statement

Reference

Actn^GD1354, Mlp84B^GD6893, Scer\GAL4^Mef2.PR has embryonic/larval hypodermal muscle cell | larval stage phenotype

(Clark and Kadrmas, 2013)

Actn^GD1354, Mlp84B^GD6893, Scer\GAL4^Mef2.PR has Z disc | larval stage phenotype

(Clark and Kadrmas, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Mlp84B^GD6893 and Actn^GD1354 under the control of Scer\GAL4^Mef2.PR in larvae causes severe disruption of muscle structure, with some wispy muscle fibers, and Z-lines that are hard to discern; these phenotypes are not seen in controls or in either single knock-down.

(Clark and Kadrmas, 2013)

Coexpression of Dcr-2^Scer\UAS.cDa with Actn^GD1354, under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF, leads to an enhancement of the Actn^GD1354 rough eye phenotype.

(Dietzl et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)