FlyBase Allele Report: Dmel\Gale[GD7464]

General Information

Symbol

Dmel\Gale^GD7464

Species

D. melanogaster

Name

FlyBase ID

FBal0199664

Feature type

allele

Associated gene

Dmel\Gale

Associated Insertion(s)

Carried in Construct

P{GD7464}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7464}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7464

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of galactosemia

CEA

(Jumbo-Lucioni et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of galactosemia

is exacerbated by Galt^Δ1AP2

(Jumbo-Lucioni et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

NMJ bouton | larval stage, with Scer\GAL4^elav.PU

(Jumbo-Lucioni et al., 2016)

Detailed Description

Statement

Reference

Larvae expressing Gale^GD7464 under the control of Scer\GAL4^elav.PU exhibit uncoordinated and slower movements in righting assays, as compared to controls; their neuromuscular junctions have significantly more synaptic boutons, but the same number of branches as controls. Expression under the control of Scer\GAL4^how-24B also leads to slower movements in righting assays, but does not cause a detectable change in synaptic architecture or a significant change in synaptic bouton number, as compared to controls.

(Jumbo-Lucioni et al., 2016)

Adults expressing Gale^GD7464 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD7464} insertion line used.

Expression of Gale^GD7464 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD7464} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 0-10% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 70-80% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 0% or 0-10% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 10-20% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development