FlyBase Allele Report: Dmel\Ncoa6[GD14752]

General Information

Symbol

Dmel\Ncoa6^GD14752

Species

D. melanogaster

Name

FlyBase ID

FBal0200568

Feature type

allele

Associated gene

Dmel\Ncoa6

Associated Insertion(s)

Carried in Construct

P{GD14752}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14752}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14752

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Oh et al., 2014)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^en.PU

(Oh et al., 2014)

visible | adult stage, with Scer\GAL4^GMR.PF

(Oh et al., 2014)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^en.PU

(Oh et al., 2014)

Detailed Description

Statement

Reference

Expression of Ncoa6^GD14752 under the control of either Scer\GAL4^GMR.PF (together with UAS-Dicer2 to enhance RNAi efficiency) or Scer\GAL4^en.PU results in smaller adult eye or wing size, respectively.

Somatic clones in the third instar larval wing disc expressing Ncoa6^GD14752 under the control of Scer\GAL4^Act.PU display increased levels of cell death (visualized by an apoptotic marker, cleaved Casp3).

(Oh et al., 2014)

Adults expressing Ncoa6^GD14752 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14752

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Ncoa6^GD14752, Scer\GAL4^GMR.PF has visible | adult stage phenotype, non-suppressible by yki^{S168A.UAS.EGFP,Tag:HA}, Scer\GAL4^GMR.PF

(Oh et al., 2014)

Ncoa6^GD14752, Scer\GAL4^GMR.PF has abnormal size | adult stage phenotype, non-suppressible by yki^{S168A.UAS.EGFP,Tag:HA}, Scer\GAL4^GMR.PF

(Oh et al., 2014)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Ncoa6^GD14752, Scer\GAL4^GMR.PF has eye | adult stage phenotype, non-suppressible by yki^{S168A.UAS.EGFP,Tag:HA}, Scer\GAL4^GMR.PF

(Oh et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The small eye phenotype characteristic for adult flies expressing Ncoa6^GD14752 under the control of Scer\GAL4^GMR.PF is not suppressed by co-expression of yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1}, which on its own lead to eye overgrowth.

(Oh et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v36480

w¹¹¹⁸; P{GD14752}v36480

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14752

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG14023^GD14752

Ncoa6^GD14752

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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