FlyBase Allele Report: Dmel\Gba1a[GD6246]

General Information

Symbol

Dmel\Gba1a^GD6246

Species

D. melanogaster

Name

FlyBase ID

FBal0202753

Feature type

allele

Associated gene

Dmel\Gba1a

Associated Insertion(s)

Carried in Construct

P{GD6246}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6246}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6246

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(Suzuki et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sleep, with Scer\GAL4^elav.PU

(Kawasaki et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of Gba1a^GD6246 under the control of Scer\GAL4^elav.PU does not lead to any significant decrease in the adult survival rate or climbing ability compared to age-matched controls but display mild sleep abnormalities.

(Kawasaki et al., 2017)

The brains of one-week old flies expressing Gba1a^GD6246 under the control of Scer\GAL4^elav-C155 exhibit an 81-92% decrease in glucocerebrosidase activity compared with controls.

(Suzuki et al., 2015)

Adults expressing Gba1a^GD6246 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 60-70% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6246

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Gba1a^GD6246, Scer\GAL4^elav-C155 is an enhancer of abnormal neuroanatomy | progressive phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Suzuki et al., 2015)

Gba1a^GD6246, Scer\GAL4^elav-C155 is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Suzuki et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Gba1a^GD6246, Scer\GAL4^elav-C155 is an enhancer of dopaminergic neuron | progressive phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Suzuki et al., 2015)

Other

Statement

Reference

Gba1a^GD6246, Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155 has retina phenotype

(Suzuki et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Gba1a^GD6246 enhances the reduction in climbing activity and loss of dopaminergic neurons seen in flies expressing Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^elav-C155.

The eyes of flies co-expressing Hsap\SNCA^Scer\UAS.cFa and Gba1a^GD6246 under the control of Scer\GAL4^GMR.PS exhibit retinal thinning. No thinning is seen when either transgene is expressed alone.

(Suzuki et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v14697

w¹¹¹⁸; P{GD6246}v14697

v14698

w¹¹¹⁸; P{GD6246}v14698