FlyBase Allele Report: Dmel\Rrp40[GD10414]

General Information

Symbol

Dmel\Rrp40^GD10414

Species

D. melanogaster

Name

FlyBase ID

FBal0203104

Feature type

allele

Associated gene

Dmel\Rrp40

Associated Insertion(s)

Carried in Construct

P{GD10414}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10414}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10414

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

exacerbates frontotemporal dementia

modeled by Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

embryonic/larval lymph gland, with Scer\GAL4^dome-PG14

(Mondal et al., 2014)

embryonic/larval lymph gland, with Scer\GAL4^HCH.Hand, Scer\GAL4^Hml.Δ

(Mondal et al., 2014)

embryonic/larval lymph gland, with Scer\GAL4^Hml.Δ

(Mondal et al., 2014)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing blade, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

Detailed Description

Statement

Reference

Expression of Rrp40^GD10414 under the control of Scer\GAL4^GMR.PU does not result in any apparent eye defects, as compared to controls.

(Freibaum et al., 2015)

Expression of Rrp40^GD10414 driven by Scer\GAL4^nub-AC-62 results in crumpled wings in 90% of adults, with the remainder having no wings.

(Towler et al., 2015)

Expression via "HHLT-gal4" or Scer\GAL4^Hml.Δ or Scer\GAL4^dome-PG14 results in loss of progenitor cells in the primary lobes of the larval lymph gland. ("HHLT-gal4" is a combination of Scer\GAL4^HCH.Hand and Scer\GAL4^Hml.Δ that express in early lymph gland blood cells (hemocytes) and circulating and sessile blood cells, respectively, and incorporates Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} to maintain expression in the lymph gland after Scer\GAL4^HCH.Hand is down-regulated.)

(Mondal et al., 2014)

Adults expressing Rrp40^GD10414 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10414

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rrp40^GD10414, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rrp40^GD10414, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Rrp40^GD10414 enhances the rough eye phenotype seen in flies expressing Zzzz\GGGGCC^{58.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU.

(Freibaum et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v40306

w¹¹¹⁸; P{GD10414}v40306

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10414

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG31938^GD10414

EXOSC3^40306/GD

(Freibaum et al., 2015)

Rrp40^GD10414

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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