FlyBase Allele Report: Dmel\EndoU[GD3933]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\EndoU^GD3933

General Information

Symbol

Dmel\EndoU^GD3933

Species

D. melanogaster

Name

FlyBase ID

FBal0203541

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

Transgenic product class

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^ChAT.7.4

(Laneve et al., 2017)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Laneve et al., 2017)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Toll-6-D42

(Laneve et al., 2017)

lethal - all die before end of P-stage, with Scer\GAL4^Act5C.PI

(Laneve et al., 2017)

partially lethal, with Scer\GAL4^elav-C155

(Laneve et al., 2017)

short lived, with Scer\GAL4^ChAT.7.4

(Laneve et al., 2017)

short lived, with Scer\GAL4^elav-C155

(Laneve et al., 2017)

short lived, with Scer\GAL4^Toll-6-D42

(Laneve et al., 2017)

uncoordinated | adult stage, with Scer\GAL4^elav-C155

(Laneve et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^ChAT.7.4

(Laneve et al., 2017)

visible | adult stage, with Scer\GAL4^elav-C155

(Laneve et al., 2017)

visible | adult stage, with Scer\GAL4^Toll-6-D42

(Laneve et al., 2017)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^ChAT.7.4

(Laneve et al., 2017)

adult cuticle, with Scer\GAL4^elav-C155

(Laneve et al., 2017)

adult thorax, with Scer\GAL4^ChAT.7.4

(Laneve et al., 2017)

lipid droplet | adult stage | decreased number, with Scer\GAL4^ppl.PP

(Sun et al., 2023)

ptilinum, with Scer\GAL4^ChAT.7.4

(Laneve et al., 2017)

ptilinum, with Scer\GAL4^elav-C155

(Laneve et al., 2017)

scutellar bristle, with Scer\GAL4^ChAT.7.4

(Laneve et al., 2017)

wing blade, with Scer\GAL4^ChAT.7.4

(Laneve et al., 2017)

wing blade, with Scer\GAL4^elav-C155

(Laneve et al., 2017)

wing blade, with Scer\GAL4^Toll-6-D42

(Laneve et al., 2017)

Detailed Description

Statement

Reference

Expression of EndoU^GD3933 under the control of Scer\GAL4^Act5C.PI results in late pupal lethality. Expression under the Scer\GAL4^GMR.PF does not have any significant effect on adult eye morphology, while Scer\GAL4^elav-C155-driven knockdown leads to increased pupal lethality, immature adult appearance with various morphological defects (unexpanded wings, unretracted ptilinum, dimpled thorax, misoriented scutellar bristles, soft cuticle) as well as dramatic reduction in adult lifespan, locomotor defects and marked lack of coordination (flies are unable to walk or stay upright). EndoU^GD3933 expression driven by either Scer\GAL4^ChAT.7.4 or Scer\GAL4^Toll-6-D42 (but not by Scer\GAL4^CCAP.PP or Scer\GAL4^Burs - these flies are viable and morphologically normal) also lead to reduced adult survival locomotion and immature morphological defects, though with the latter driver the phenotypes are much less severe and the morphological defects infrequent.

(Laneve et al., 2017)

Adults expressing CG3303^GD3933 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3933

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

EndoU^GD3933, Scer\GAL4^ppl.PP has lipid droplet | adult stage | decreased number phenotype, non-enhanceable by Arlr²⁶²

(Sun et al., 2023)

Suppressed by

Statement

Reference

EndoU^GD3933, Scer\GAL4^ppl.PP has lipid droplet | adult stage | decreased number phenotype, suppressible by Arlr^UAS.Tag:HA, Scer\GAL4^ppl.PP

(Sun et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

v9916

w¹¹¹⁸; P{GD3933}v9916

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3933

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG3303^GD3933

EndoU^GD3933

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections