FlyBase Allele Report: Dmel\Tmtc3[GD2121]

General Information

Symbol

Dmel\Tmtc3^GD2121

Species

D. melanogaster

Name

FlyBase ID

FBal0204155

Feature type

allele

Associated gene

Dmel\Tmtc3

Associated Insertion(s)

Carried in Construct

P{GD2121}

Also Known As

UAS-tmtc3-IR1

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2121}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2121

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of epilepsy

CEA

(Farhan et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of epilepsy

is ameliorated by Hsap\TMTC3^UAS.cFa

(Farhan et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

paralytic | adult stage, with Scer\GAL4^elav.PU

(Farhan et al., 2017)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Abnormally high proportion of adult flies expressing Tmtc3^GD2121 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^elav.PU display seizure-like behavior and remain temporally immobilized in response to mechanical disturbance (vortexing). However, the severity of the seizure (duration of immobilization) is not significantly different from seizure-experiencing controls. No obvious morphological defects in neuronal structures (mushroom body, dendrite arborization in type IV multidendrite neurons, synaptic morphology at neuromuscular junction) are observed in Tmtc3^GD2121 knock-down flies (with Scer\GAL4⁴⁷⁷ or Scer\GAL4^elav.PU driver)

(Farhan et al., 2017)

Adults expressing CG4050^GD2121 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2121

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, Tmtc3^GD2121 has paralytic | adult stage phenotype, suppressible by Hsap\TMTC3^UAS.cFa, Scer\GAL4^elav.PU

(Farhan et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The susceptibility to seizures induced by mechanical stress (flies remain immobilized after vortexing) characteristic for adult flies expressing Tmtc3^GD2121 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^elav.PU is rescued by co-expression of Hsap\TMTC3^Scer\UAS.cFa.

(Farhan et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v33248

w¹¹¹⁸; P{GD2121}v33248

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2121

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG4050^GD2121

Tmtc3^GD2121

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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