FlyBase Allele Report: Dmel\Flacc[GD12212]

General Information

Symbol

Dmel\Flacc^GD12212

Species

D. melanogaster

Name

FlyBase ID

FBal0205436

Feature type

allele

Associated gene

Dmel\Flacc

Associated Insertion(s)

Carried in Construct

P{GD12212}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12212}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12212

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal sex-determination, with Scer\GAL4^dome-PG14

(Guo et al., 2018, Knuckles et al., 2018)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of Flacc^GD12212 under the control of Scer\GAL4^dome-PG14 leads to partial sex transformation in genetically female flies: the adult females develop sex combs on the forelegs and display severe genitalia defects - absence of typical female strutures (vaginal bristles) and presence of structures resembling the male penis apparatus.

(Guo et al., 2018)

Adult females expressing Flacc^GD12212 under the control of Scer\GAL4^dome-PG14 display sex-determination defects manifested by the appearance of sex combs on their forelegs.

(Knuckles et al., 2018)

Expression of CG7358^GD12212 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver results in viable flies or partial lethality, depending on the particular P{GD12212} insertion line used.

(Neely et al., 2010)

Adults expressing CG7358^GD12212 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD12212} insertion line used.

Adults expressing CG7358^GD12212 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in an enlarged notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12212

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v35209

w¹¹¹⁸; P{GD12212}v35209

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12212

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG7358^GD12212

Flacc^GD12212

xio^GD12212

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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