FlyBase Allele Report: Dmel\Seipin[GD3208]

General Information

Symbol

Dmel\Seipin^GD3208

Species

D. melanogaster

Name

FlyBase ID

FBal0206404

Feature type

allele

Associated gene

Dmel\Seipin

Associated Insertion(s)

Carried in Construct

P{GD3208}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3208}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3208

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of congenital generalized lipodystrophy

CEA

(Bi et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

fat body, with Scer\GAL4^ppl.PP

(Tian et al., 2011)

lipid droplet, with Scer\GAL4^ppl.PP

(Tian et al., 2011)

lipid droplet | ectopic, with Scer\GAL4^48Y

(Tian et al., 2011)

lipid droplet | ectopic, with Scer\GAL4^elav.PU

(Tian et al., 2011)

lipid droplet | ectopic, with Scer\GAL4^ppl.PP

(Tian et al., 2011)

salivary gland, with Scer\GAL4^48Y

(Tian et al., 2011)

salivary gland, with Scer\GAL4^elav.PU

(Tian et al., 2011)

salivary gland, with Scer\GAL4^ppl.PP

(Tian et al., 2011)

Detailed Description

Statement

Reference

Expression of Seipin^GD3208 using Scer\GAL4^ppl.PP results in ectopic lipid storage in the salivary gland with mild lipid storage defects in the fat body.

Expression of Seipin^GD3208 using Scer\GAL4^Sgs3.PD does not result in ectopic lipid storage in the salivary gland, whereas expression using Scer\GAL4^48Y or Scer\GAL4^elav.PU does produce this phenotype.

(Tian et al., 2011)

Adults expressing Seipin^GD3208 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3208

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ppl.PP, Seipin^GD3208 has adult fat body phenotype, enhanceable by SERCA^GD436, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Scer\GAL4^ppl.PP, Seipin^GD3208 has lipid droplet phenotype, enhanceable by SERCA^GD436, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Enhancer of

Statement

Reference

Seipin^GD3208, Scer\GAL4^ppl.PP is an enhancer of adult fat body phenotype of SERCA^GD436, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Seipin^GD3208, Scer\GAL4^ppl.PP is an enhancer of lipid droplet phenotype of SERCA^GD436, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Seipin^GD3208 and Ca-P60A^GD436 under the control of Scer\GAL4^ppl.PP further enhances the reduction in lipid droplet size and decrease in glyceride levels seen when either transgene is expressed alone.

(Bi et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v45478

w¹¹¹⁸; P{GD3208}v45478

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3208

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG9904^GD3208

Seipin^GD3208

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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