FlyBase Allele Report: Dmel\CycJ[GD6936]

General Information

Symbol

Dmel\CycJ^GD6936

Species

D. melanogaster

Name

FlyBase ID

FBal0206713

Feature type

allele

Associated gene

Dmel\CycJ

Associated Insertion(s)

Carried in Construct

P{GD6936}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6936}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6936

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage

(Lee et al., 2016)

partially lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

some die during pupal stage, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult anterior midgut epithelium | conditional, with Scer\GAL4^NP3084

(Lee et al., 2016)

Detailed Description

Statement

Reference

Adult flies expressing CycJ^GD6936 under the control of Scer\GAL4^NP3084 (together with tub-Gal80[ts] to limit the time of expression) display normal midgut epithelium thinning upon infection with Serratia marcescens but in contrast to wild-type the epithelium fails to recover and the flies have reduced infection survival rate. However, in mosaic midguts bearing Scer\GAL4^esg-NP7397-induced flip-out clones expressing CycJ^GD6936 under the Scer\GAL4^Act5C.PP driver, the knockdown enterocytes recover as well as the wild-type enterocytes in the same gut.

(Lee et al., 2016)

Adults expressing CycJ^GD6936 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development