FlyBase Allele Report: Dmel\Dsk[GD6123]

General Information

Symbol

Dmel\Dsk^GD6123

Species

D. melanogaster

Name

FlyBase ID

FBal0206910

Feature type

allele

Associated gene

Dmel\Dsk

Associated Insertion(s)

Carried in Construct

P{GD6123}

Also Known As

UAS-dsk-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6123}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6123

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Söderberg et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage, with Scer\GAL4^Dsk.PP

(Söderberg et al., 2012)

abnormal chemosensory behavior | adult stage, with Scer\GAL4^Ilp2.215-3

(Söderberg et al., 2012)

abnormal chemosensory behavior | larval stage, with Scer\GAL4^Dsk.PP

(Söderberg et al., 2012)

abnormal chemosensory behavior | larval stage, with Scer\GAL4^Ilp2.215-3

(Söderberg et al., 2012)

abnormal feeding behavior | adult stage, with Scer\GAL4^Dsk.PP

(Söderberg et al., 2012)

abnormal feeding behavior | adult stage, with Scer\GAL4^Ilp2.215-3

(Söderberg et al., 2012)

abnormal feeding behavior | larval stage, with Scer\GAL4^Dsk.PP

(Söderberg et al., 2012)

abnormal feeding behavior | larval stage, with Scer\GAL4^Ilp2.215-3

(Söderberg et al., 2012)

abnormal locomotor behavior | larval stage, with Scer\GAL4^Dsk.PP

(Söderberg et al., 2012)

abnormal locomotor behavior | larval stage, with Scer\GAL4^Ilp2.215-3

(Söderberg et al., 2012)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^c739

(Chen and Ganetzky, 2012)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Chen and Ganetzky, 2012)

abnormal starvation stress response | adult stage, with Scer\GAL4^Dsk.PP

(Söderberg et al., 2012)

abnormal starvation stress response | adult stage, with Scer\GAL4^Ilp2.215-3

(Söderberg et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^c739

(Chen and Ganetzky, 2012)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

(Chen and Ganetzky, 2012)

NMJ bouton | third instar larval stage, with Scer\GAL4^c739

(Chen and Ganetzky, 2012)

NMJ bouton | third instar larval stage, with Scer\GAL4^elav-C155

(Chen and Ganetzky, 2012)

Detailed Description

Statement

Reference

Expression of Dsk^GD6123 under the control of Scer\GAL4^nSyb.PU (in combination with UAS-Dicer2 to increase RNAi efficiency) does not result in a significant change in the latency to sperm ejection in females after the end of copulation.

(Lee et al., 2015)

Expression of Dsk^GD6123 in larvae using Scer\GAL4^elav-C155 results in neuromuscular junction (NMJ) undergrowth, compared with wild-type.

Expression of Dsk^GD6123 under the control of Scer\GAL4^Rapgap1-OK6 does not cause NMJ undergrowth.

Expression of Dsk^GD6123 under the control of Scer\GAL4^c739 results in significant NMJ undergrowth.

(Chen and Ganetzky, 2012)

Expression of Dsk^GD6123 under the control of Scer\GAL4^Ilp2.215-3 or Scer\GAL4^Dsk.PP in adult males significantly increases feeding following starvation, and significantly increases feeding on undesirable food in a choice assay, compared to controls; adult males also have significantly increased survival under starvation conditions, compared to controls; expression in larvae also significantly increases food intake, and significantly increases numbers remaining on undesirable food in a choice assay, compared to controls.

(Söderberg et al., 2012)

Adults expressing Dsk^GD6123 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6123

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v14201

w¹¹¹⁸; P{GD6123}v14201

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6123

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Dsk^GD6123

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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