FlyBase Allele Report: Dmel\Fit1[GD17050]

General Information

Symbol

Dmel\Fit1^GD17050

Species

D. melanogaster

Name

FlyBase ID

FBal0207037

Feature type

allele

Associated gene

Dmel\Fit1

Associated Insertion(s)

Carried in Construct

P{GD17050}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD17050}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD17050

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Shulman et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

17050

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Fit1^GD17050, Scer\GAL4^Hand.PA has cardial cell | adult stage phenotype, enhanceable by Fit2^GD1300, Scer\GAL4^Hand.PA

(Catterson et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of P{GD17050} (which is predicated to target Fit2 as a secondary target, as well as Fit1) under the control of Scer\GAL4^Hand.PA leads to the loss of the cardiac syncytium in the adult heart. The distance between cardiomyocytes is ten-fold greater than in controls. Heart beat rate is not different from wild type, but the fractional shortening (distance the heart moves between diastole and systole) is greatly reduced and the end systolic diameter is increased compared to wild type. The mutant heart rhythm shows a loss of synchrony between cardiomyocytes.

Co-expression of P{GD17050} and Fit2^GD1300 under the control of Scer\GAL4^Hand.PA results in a severe defect in the adult heart muscle, with the cardiomyocytes showing a 'rounded-up' phenotype.

(Catterson et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v46494