FlyBase Allele Report: Dmel\Idgf1[GD5405]

General Information

Symbol

Dmel\Idgf1^GD5405

Species

D. melanogaster

Name

FlyBase ID

FBal0207362

Feature type

allele

Associated gene

Dmel\Idgf1

Associated Insertion(s)

Carried in Construct

P{GD5405}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5405}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5405

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal wound healing, with Scer\GAL4^69B

(Pesch et al., 2016)

partially lethal, with Scer\GAL4^69B

(Behr and Riedel, 2020, Pesch et al., 2016)

partially lethal - majority live, with Scer\GAL4^btl.PS

(Behr and Riedel, 2020)

some die during larval stage, with Scer\GAL4^69B

(Pesch et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

embryonic/larval cuticle, with Scer\GAL4^69B

(Pesch et al., 2016)

Detailed Description

Statement

Reference

Under control conditions, the adulthood-only expression of Idgf1^GD5405 under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) does not significantly affect the mitotic index in the female or male adult posterior midgut, as compared to controls.

(Hudry et al., 2016)

Scer\GAL4^69B-mediated expression results in larval cuticle molting defects - mutants show characteristic double-cuticles of head skeleton and posterior spiracles in the second and third instar larvae.

Scer\GAL4^69B-mediated expression results in large epidermal lesions at wounded sites of first instar and immediate lethality.

Scer\GAL4^69B-mediated expression results in epidermal cuticle morphology defects in third instars - unusually narrowed ECM thickness and deformed chitin-matrix, and absence of lamellate chitin-scaffolds.

(Pesch et al., 2016)

Adults expressing Idgf1^GD5405 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development