FlyBase Allele Report: Dmel\ImpL2[GD6004]

General Information

Symbol

Dmel\ImpL2^GD6004

Species

D. melanogaster

Name

FlyBase ID

FBal0207380

Feature type

allele

Associated gene

Dmel\ImpL2

Associated Insertion(s)

Carried in Construct

P{GD6004}

Also Known As

UAS-Impl2^RNAi, ImpL2^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6004}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6004

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.cUa.UAS, dlg1^RNAi.UAS.cUa

(Lodge et al., 2021)

modeled by Ras85D^V12.UAS, dlg1^GD4689

(Figueroa-Clarevega and Bilder, 2015)

modeled by yki^{S168A.UAS.EGFP,Tag:HA}

(Yeom et al., 2021)

modeled by Ras85D^V12.UAS, dlg1^RNAi.UAS.cUa

(Bakopoulos et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of ImpL2^NIG.15009R in adult intestinal stem cells (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) has no effect on either circulating or whole-body trehalose levels.

(Kwon et al., 2015)

Adults expressing ImpL2^GD6004 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6004

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ImpL2^GD6004, Scer\GAL4^ey.PU is a suppressor | partially of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^ey.PU, dlg1^GD4689

(Figueroa-Clarevega and Bilder, 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

ImpL2^GD6004, Scer\GAL4^Act5C.PP is a suppressor | partially of larval muscle system | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^RNAi.UAS.cUa

(Bakopoulos et al., 2023)

ImpL2^GD6004/gbb^VSH330684, Scer\GAL4^Act5C.PP is a suppressor of larval muscle system | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^RNAi.UAS.cUa

(Bakopoulos et al., 2023)

ImpL2^GD6004/gbb^VSH330684, Scer\GAL4^Act5C.PP is a suppressor | partially of embryonic/larval fat body | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^RNAi.UAS.cUa

(Bakopoulos et al., 2023)

ImpL2^GD6004, Scer\GAL4^Act5C.PP is a suppressor | partially of embryonic/larval fat body | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^RNAi.UAS.cUa

(Bakopoulos et al., 2023)

ImpL2^GD6004/gbb^VSH330684, Scer\GAL4^Act5C.PP is a suppressor | partially of extracellular matrix | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^RNAi.UAS.cUa

(Bakopoulos et al., 2023)

ImpL2^GD6004, Scer\GAL4^Act5C.PP is a suppressor | partially of muscle attachment site | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^RNAi.UAS.cUa

(Bakopoulos et al., 2023)

ImpL2^GD6004/gbb^VSH330684, Scer\GAL4^Act5C.PP is a suppressor of muscle attachment site | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^RNAi.UAS.cUa

(Bakopoulos et al., 2023)

NOT Suppressor of

Statement

Reference

ImpL2^GD6004, Scer\GAL4^Act5C.PP is a non-suppressor of extracellular matrix | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^RNAi.UAS.cUa

(Bakopoulos et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ImpL2^GD6004 within the tumors of Scer\GAL4^ey.PU>Ras85D^V12.Scer\UAS,dlg1^GD4689 origin significantly ameliorates the peripheral tissue wasting phenotypes. Hosts bearing dlg1^GD4689, Ras85D^V12.Scer\UAS, ImpL2^GD6004 tumors show increased abdominal fat body mass. Muscle function assays further reveal improvements in both climbing ability and speed. There is a significant rescue of ovariole health, leading to restoration of egg production.

(Figueroa-Clarevega and Bilder, 2015)

Expression of ImpL2^GD6004 partially suppresses the increase in circulating and whole-body trehalose levels seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B). The overall shape of the midguts is unchanged.

(Kwon et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v30930

w¹¹¹⁸; P{GD6004}v30930

v30931

w¹¹¹⁸; P{GD6004}v30931/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6004

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ImpL2^GD6004

ImpL2^RNAi

(Figueroa-Clarevega and Bilder, 2015)

Impl2^Ri

(Lodge et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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