FlyBase Allele Report: Dmel\Kmn1[GD9062]

General Information

Symbol

Dmel\Kmn1^GD9062

Species

D. melanogaster

Name

FlyBase ID

FBal0207456

Feature type

allele

Associated gene

Dmel\Kmn1

Associated Insertion(s)

Carried in Construct

P{GD9062}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9062}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9062

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain without any change in size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing Kmn1^GD9062 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9062

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

BacA\p35^UAS.cUa, Kmn1^GD9062, Scer\GAL4^ap.PU has abnormal endomitotic cell cycle phenotype

(Clemente-Ruiz et al., 2014)

BacA\p35^UAS.cUa, Kmn1^GD9062, Scer\GAL4^ap.PU has neoplasia phenotype

(Clemente-Ruiz et al., 2014)

BacA\p35^UAS.cUa, Kmn1^GD9062, Scer\GAL4^en.PU has abnormal endomitotic cell cycle phenotype

(Clemente-Ruiz et al., 2014)

BacA\p35^UAS.cUa, Kmn1^GD9062, Scer\GAL4^en.PU has neoplasia phenotype

(Clemente-Ruiz et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Kmn1^GD9062 and BacA\p35^Scer\UAS.cUa in the dorsal compartment of the wing disc, under the control of Scer\GAL4^ap.PU, results in a high percentage of cells with DNA content higher than 4n (up to 30%) when compared to control cells. This is accompanied by some tissue overgrowth, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35^Scer\UAS.cUa-expressing control larvae and ultimately die.

Expression of Kmn1^GD9062 and BacA\p35^Scer\UAS.cUa in the posterior compartment of the wing disc, under the control of Scer\GAL4^en.PU, results in some tissue overgrowth. The resulting wing primordia are massively overgrown, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35^Scer\UAS.cUa-expressing control larvae and ultimately die.

(Clemente-Ruiz et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v19529

w¹¹¹⁸; P{GD9062}v19529

Notes on Origin

Discoverer