FlyBase Allele Report: Dmel\Mkp3[GD8219]

General Information

Symbol

Dmel\Mkp3^GD8219

Species

D. melanogaster

Name

FlyBase ID

FBal0207624

Feature type

allele

Associated gene

Dmel\Mkp3

Associated Insertion(s)

Carried in Construct

P{GD8219}

Also Known As

UAS-Mkp3-i

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8219}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8219

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^da.G32

(Ha et al., 2009)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

short lived, with Scer\GAL4^da.G32

(Ha et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult posterior midgut epithelium, with Scer\GAL4^da.G32

(Ha et al., 2009)

Detailed Description

Statement

Reference

Adults expressing Mkp3^GD8219 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Animals expressing Mkp3^GD8219 under the control of Scer\GAL4^da.G32 have a significantly shorter life span than controls. Posterior midgut cells in these animals show a high level of apoptosis.

(Ha et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 10-20% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8219

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mkp3^GD8219, Scer\GAL4^da.G32 has short lived phenotype, suppressible by Duox^RNAi.UAS, Scer\GAL4^da.G32

(Ha et al., 2009)

Mkp3^GD8219, Scer\GAL4^da.G32 has increased cell death phenotype, suppressible by Duox^RNAi.UAS, Scer\GAL4^da.G32

(Ha et al., 2009)

Enhancer of

Statement

Reference

Mkp3^GD8219, Scer\GAL4^MD-638 is an enhancer of visible | adult stage phenotype of Scer\GAL4^MD-638, tay^RNAi.UAS

(Molnar and de Celis, 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Mkp3^GD8219, Scer\GAL4^da.G32 has adult posterior midgut epithelium phenotype, suppressible by Duox^RNAi.UAS, Scer\GAL4^da.G32

(Ha et al., 2009)

Enhancer of

Statement

Reference

Mkp3^GD8219, Scer\GAL4^MD-638 is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^MD-638, tay^RNAi.UAS

(Molnar and de Celis, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Mkp3^GD8219 enhances the wing vein phenotype seen when tay^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^MD-638.

(Molnar and de Celis, 2013)

The shortened lifespan and increased apoptosis seen in the posterior midgut of flies expressing Mkp3^GD8219 under the control of Scer\GAL4^da.G32 is significantly rescued by co-expression of Duox^{dsRNA.Scer\UAS}.

(Ha et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v23911