UASt regulatory sequences drive expression of an inverted repeat.
Expression of MntGD4469 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver results in viable flies or complete lethality, depending on the particular P{GD4469} insertion line used.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or early pupal lethality.
Expression under the control of Scer\GAL4pnr-MD237 may result in lethality before the pupal stage, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 50-60% of the Scer\GAL4pnr-MD237-expressing area of the notum.