FlyBase Allele Report: Dmel\Nedd4[GD5146]

General Information

Symbol

Dmel\Nedd4^GD5146

Species

D. melanogaster

Name

FlyBase ID

FBal0207730

Feature type

allele

Associated gene

Dmel\Nedd4

Associated Insertion(s)

Carried in Construct

P{GD5146}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5146}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5146

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Nedd4^GD5146 under the control of Scer\GAL4^GMR.PU at 28[o]C does not affect eye development.

Expression of Nedd4^GD5146 under the control of Scer\GAL4^elav.PU does not affect climbing ability or lifespan.

(Davies et al., 2014)

Adults expressing Nedd4^GD5146 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5146

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Nedd4^GD5146, Scer\GAL4^elav.PU is an enhancer of abnormal locomotor behavior phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav.PU

(Davies et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

Nedd4^GD5146, Scer\GAL4^elav.PU is an enhancer of dopaminergic neuron phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav.PU

(Davies et al., 2014)

NOT Enhancer of

Statement

Reference

Nedd4^GD5146, Scer\GAL4^GMR.PU is a non-enhancer of eye | heat sensitive phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Davies et al., 2014)

NOT Suppressor of

Statement

Reference

Nedd4^GD5146, Scer\GAL4^GMR.PU is a non-suppressor of eye | heat sensitive phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Davies et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Knockdown of Nedd4, through expression of Nedd4^GD5146 does not affect the rough eye phenotype induced by Hsap\SNCA^Scer\UAS.cFa in the developing eye under the control of Scer\GAL4^GMR.PU.

Co-expression of Nedd4^{Scer\UAS.T:Hsap\MYC} prevents the climbing deficit associated with neuronal Hsap\SNCA^Scer\UAS.cFa expression (both constructs under the control of Scer\GAL4^elav.PU).

Knockdown of Nedd4, through expression of Nedd4^GD5146, combined with expression of Hsap\SNCA^Scer\UAS.cFa, in the nervous system (under the control of Scer\GAL4^elav.PU) leads to a significant reduction in the number of ple-positive neurons in the dorsomedial cluster when compared to controls.

(Davies et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v13121

w¹¹¹⁸; P{GD5146}v13121/TM3

Notes on Origin

Discoverer