FlyBase Allele Report: Dmel\Nmnat[GD8082]

General Information

Symbol

Dmel\Nmnat^GD8082

Species

D. melanogaster

Name

FlyBase ID

FBal0207767

Feature type

allele

Associated gene

Dmel\Nmnat

Associated Insertion(s)

Carried in Construct

P{GD8082}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8082}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8082

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone, with Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

axon | adult stage | somatic clone, with Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

neuronal cell body | adult stage | somatic clone, with Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

sensory neuron | adult stage | somatic clone, with Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

Detailed Description

Statement

Reference

Expressing Nmnat^GD8082 under the control of Scer\GAL4^tey-5053A (together with Dicer-2, for efficient RNAI) does not provide protection against (injury-induced) Wallerian degeneration of third instar larval motoneurons.

(Hao et al., 2019)

Sensory neuron clones in the adult wing expressing Nmnat^GD8082 under the control of Scer\GAL4^VGlut-OK371 display axon and cell body degeneration.

(Neukomm et al., 2017)

Adults expressing Nmnat^GD8082 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8082

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by axed⁰⁰¹¹

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by axed²⁰⁹⁴

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible | partially by Sarm⁸⁹⁶

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by axed²⁰⁹⁴/Sarm⁸⁹⁶

(Neukomm et al., 2017)

NOT suppressed by

Statement

Reference

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | somatic clone | adult stage phenotype, non-suppressible by hiw^ΔN

(Neukomm et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^tey-5053A/Nmnat^GD8082 is a non-suppressor of abnormal neuroanatomy | recessive | third instar larval stage | conditional phenotype of raw^dcp-1

(Hao et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has sensory neuron | somatic clone | adult stage phenotype, suppressible by axed²⁰⁹⁴

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has axon | somatic clone | adult stage phenotype, suppressible by axed²⁰⁹⁴

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has neuronal cell body | somatic clone | adult stage phenotype, suppressible by axed²⁰⁹⁴

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has sensory neuron | somatic clone | adult stage phenotype, suppressible | partially by Sarm⁸⁹⁶

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has axon | somatic clone | adult stage phenotype, suppressible | partially by Sarm⁸⁹⁶

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has neuronal cell body | somatic clone | adult stage phenotype, suppressible | partially by Sarm⁸⁹⁶

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has sensory neuron | somatic clone | adult stage phenotype, suppressible by axed²⁰⁹⁴/Sarm⁸⁹⁶

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has neuronal cell body | somatic clone | adult stage phenotype, suppressible by axed²⁰⁹⁴/Sarm⁸⁹⁶

(Neukomm et al., 2017)

Nmnat^GD8082 has axon | somatic clone | adult stage phenotype, suppressible by axed²⁰⁹⁴/Sarm⁸⁹⁶

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has sensory neuron | somatic clone | adult stage phenotype, suppressible by axed⁰⁰¹¹

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has axon | somatic clone | adult stage phenotype, suppressible by axed⁰⁰¹¹

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has neuronal cell body | somatic clone | adult stage phenotype, suppressible by axed⁰⁰¹¹

(Neukomm et al., 2017)

NOT suppressed by

Statement

Reference

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has sensory neuron | somatic clone | adult stage phenotype, non-suppressible by hiw^ΔN

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has axon | somatic clone | adult stage phenotype, non-suppressible by hiw^ΔN

(Neukomm et al., 2017)

Nmnat^GD8082, Scer\GAL4^VGlut1-OK371 has neuronal cell body | somatic clone | adult stage phenotype, non-suppressible by hiw^ΔN

(Neukomm et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^tey-5053A/Nmnat^GD8082 is a non-suppressor of axon | third instar larval stage | conditional phenotype of raw^dcp-1

(Hao et al., 2019)

Scer\GAL4^tey-5053A/Nmnat^GD8082 is a non-suppressor of embryonic/larval motor neuron | third instar larval stage | conditional phenotype of raw^dcp-1

(Hao et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

raw^dcp-1 homozygotes show protection against (injury-induced) Wallerian degeneration of third instar larval motor, which is only very slightly suppressed by the expression of Nmnat^GD8082 under the control of Scer\GAL4^tey-5053A (together with Dicer-2, for efficient RNAI).

(Hao et al., 2019)

The axon and cell body degeneration in sensory neuron clones in the adult wing expressing Nmnat^GD8082 under the control of Scer\GAL4^VGlut-OK371 can be fully rescued by combination with axed⁰⁰¹¹, axed²⁰⁹⁴ or by axed²⁰⁹⁴ and Ect4⁸⁹⁶ together, partially so by combination with Ect4⁸⁹⁶ alone and is only marginally improved by hiw^ΔN.

(Neukomm et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v32255

w¹¹¹⁸; P{GD8082}v32255

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8082

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nmnat^GD8082

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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