FlyBase Allele Report: Dmel\Rae1[GD14705]

General Information

Symbol

Dmel\Rae1^GD14705

Species

D. melanogaster

Name

FlyBase ID

FBal0208230

Feature type

allele

Associated gene

Dmel\Rae1

Associated Insertion(s)

Carried in Construct

P{GD14705}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14705}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14705

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates acute myeloid leukemia

modeled by Hsap\HOXA9::Hsap\NUP98^WT.UAS

(Gavory et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle, with Dcr-2^UAS.cDa, Scer\GAL4^VP16.bam

(Volpi et al., 2013)

abnormal mitotic cell cycle, with Scer\GAL4^wor.PA

(Volpi et al., 2013)

male sterile, with Dcr-2^UAS.cDa, Scer\GAL4^VP16.bam

(Volpi et al., 2013)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Nebenkern, with Dcr-2^UAS.cDa, Scer\GAL4^VP16.bam

(Volpi et al., 2013)

onion stage spermatid, with Dcr-2^UAS.cDa, Scer\GAL4^VP16.bam

(Volpi et al., 2013)

primary spermatocyte, with Dcr-2^UAS.cDa, Scer\GAL4^VP16.bam

(Volpi et al., 2013)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of Rae1^GD14705 under the control of Scer\GAL4^{bam.T:Hsim\VP16} (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi) results in male sterility. Primary spermatocytes contain nuclear herniations, onion stage spermatids have nuclei and Nebenkern of varying sizes and spermatid bundles contain round, scattered and irregularly sized nuclei. Meiotic defects are seen, including irregular metaphase plates in metaphase I and lagging chromosomes and unbalanced chromosome segregation in anaphase I.

Brain neuroblasts of third instar larvae expressing Rae1^GD14705 under the control of Scer\GAL4^wor.PA have a significantly higher mitotic index compared to wild type. 70% of anaphase figures have defects, including lagging chromosomes, chromatin bridges and uneven chromosome segregation.

(Volpi et al., 2013)

Adults expressing Rae1^GD14705 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 60-70% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 30-40% or 90-100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development