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General Information
Symbol
Dmel\Rho1GD4726
Species
D. melanogaster
Name
FlyBase ID
FBal0208281
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Hemocytes trans-migrate properly from the head to tail in embryos expressing Rho1GD4726 under the control of Scer\GAL4Pxn.PS. However, fewer of the tail hemocytes migrate anteriorly along the midline in the knock-down line than in wild-type.

Compared with wild-type, posterior hemocytes in Scer\GAL4Pxn.PS>Rho1GD4726 embryos exhibit severe reduction in cellular protrusion area. The anterior hemocytes of the same genotype also show a moderate reduction in cellular protrusion area. The number of vacuoles present in both the anterior and posterior hemocyte cell bodies is increased relative to wild-type.

Eye-antennal disc clones expressing Rho1GD4726 under the control of Scer\GAL4tub.PU exhibit defects in differentiation and cell morphology, but clone size is not significantly affected as compared to control clones.

Expression under the control of Scer\GAL4Mef2.PR results in late larval lethality.

Expression of Rho1GD4726 under the control of Scer\GAL4ppl.PP results in animals with a dorsal cleft in the abdominal cuticle (most of these animals die in the puparium, with approximately 12% eclosing).

Apical F-actin in the trachea disappears in embryos expressing Rho1GD4726 under the control of Scer\GAL4btl.PS.

Expression of Rho1GD4726 in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B) results in highly elongated apical-basal cell length in mutant cells compared with control cells.

Cells in the wing expressing Rho1GD4726 under the control of Scer\GAL4ptc-559.1 (the flies also carry Scer\GAL80ts.αTub84B but it has been inactivated by shifting to 29[o]C) often produce multiple hairs (cells without any hair are also seen).

Animals carrying Rho1GD4726, Scer\GAL4ptc-559.1 and Scer\GAL80ts.αTub84B which have been shifted to 29[o]C for 26-36 hours to inactivate Scer\GAL80ts.αTub84B and allow expression of Rho1GD4726 under the control of Scer\GAL4ptc-559.1 (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency) have pupal wings with cells that have a decreased height, increased cross sectional area and decreased F-actin. The dorsal and ventral layers of the wing separate basally leaving an internal hole.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Rho1GD4726 suppresses the small clone size, differentiation and cell morphology defects seen in eye-antennal disc clones expressing RhoGEF2RE.Scer\UAS under the control of Scer\GAL4tub.PU.

Co-expression of Rho1GD4726 suppresses the formation of undifferentiated clonal masses and partially suppresses the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2RE.Scer\UAS and RafScer\UAS.F179 under the control of Scer\GAL4tub.PU, and also suppresses the developmental delay shown by larvae containing these clones.

Co-expression of Rho1GD4726 fails to suppress the ectopic differentiation of seen in eye-antennal disc clones expressing RafScer\UAS.F179 under the control of Scer\GAL4tub.PU.

Co-expression of Atf3Scer\UAS.cSa and Rho1GD4726 under the control of Scer\GAL4ppl.PP results in a more severe phenotype than co-expression of either construct alone; no animals eclose and the defect in the dorsal abdomen becomes more severe.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Rho1GD4726
Name Synonyms
Secondary FlyBase IDs
    References (11)