FlyBase Allele Report: Dmel\Rox8[GD11645]

General Information

Symbol

Dmel\Rox8^GD11645

Species

D. melanogaster

Name

FlyBase ID

FBal0208320

Feature type

allele

Associated gene

Dmel\Rox8

Associated Insertion(s)

Carried in Construct

P{GD11645}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11645}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11645

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Ras85D^V12.UAS

(Guo et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | larval stage | somatic clone, with Scer\GAL4^en.PU

(Guo et al., 2020)

increased size, with Scer\GAL4^esg.PU

(Guo et al., 2020)

increased size | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Guo et al., 2020)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

midgut, with Scer\GAL4^esg.PU

(Guo et al., 2020)

wing disc | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Guo et al., 2020)

wing disc | larval stage | somatic clone, with Scer\GAL4^en.PU

(Guo et al., 2020)

Detailed Description

Statement

Reference

Eye-antennal disc clones that express Rox8^GD11645 under the control of Scer\GAL4^Act.PU are larger than controls.

The expression of Rox8^GD11645 under the control of Scer\GAL4^en.PU results in an increased mitotic index in the wing disc posterior compartment.

The expression of Rox8^GD11645 under the control of Scer\GAL4^esg.PU leads to enlarged guts with increased mitotic index.

(Guo et al., 2020)

Adults expressing Rox8^GD11645 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development