Larvae expressing RpA-70^GD4228 under the control of Scer\GAL4^c768 show gradual loss of neuroepithelium cells of the optic lobe during the early to mid third instar, and by the late third instar, very few neuroepithelium cells remain in the mutant brain lobe. The lamina of the optic lobe is missing and the medulla cortex is reduced in size in mutant late third instar larvae.

Clones in the outer proliferation center (OPC) neuroepithelium expressing RpA-70^GD4228 under the control of Scer\GAL4^Act.PU are small and composed of only a few cells. The cells retain columnar neuroepithelium cell morphology. Some of the mutant cells are extruded from the neuroepithelium and basally enter the medulla cortex. These extruded cells change morphology and become rounded or irregular in shape.

Adults expressing RpA-70^GD4228 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD4228} insertion line used.

Adults expressing RpA-70^GD4228 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

Expression of RpA-70^GD4228 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD4228} insertion line used.

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% of the Scer\GAL4^pnr-MD237-expressing area of the notum.