FlyBase Allele Report: Dmel\Rtnl1[GD900]

General Information

Symbol

Dmel\Rtnl1^GD900

Species

D. melanogaster

Name

FlyBase ID

FBal0208428

Feature type

allele

Associated gene

Dmel\Rtnl1

Associated Insertion(s)

Carried in Construct

P{GD900}

Also Known As

UAS-Rtnl1-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD900}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD900

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of hereditary spastic paraplegia

CEA

(Fowler and O'Sullivan, 2016, O'Sullivan et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^da.G32

(Fowler and O'Sullivan, 2016, O'Sullivan et al., 2012)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Summerville et al., 2016)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^elav-C155

(Summerville et al., 2016)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^Gli.PU

(Summerville et al., 2016)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^Mef2.PR

(Summerville et al., 2016)

short lived, with Scer\GAL4^da.G32

(Fowler and O'Sullivan, 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^da.G32

(O'Sullivan et al., 2012)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

axon | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

(Summerville et al., 2016)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Gli.PU

(Summerville et al., 2016)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Mef2.PR

(Summerville et al., 2016)

endoplasmic reticulum, with Scer\GAL4^RapGAP1-OK6

(O'Sullivan et al., 2012)

mitochondrion, with Scer\GAL4^RapGAP1-OK6

(O'Sullivan et al., 2012)

mitochondrion | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

motor neuron | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

NMJ bouton | third instar larval stage, with Scer\GAL4^elav-C155

(Summerville et al., 2016)

Detailed Description

Statement

Reference

Expression of Rtnl1^GD900 under the control of Scer\GAL4^da.G32 results in a more rapid age-progressive decline in climbing abilities of adult flies and mild decrease in their lifespan compared to controls. Expression under the Scer\GAL4^RapGAP1-OK6 driver leads to defects in mitochondrial morphology in the distal portions of longer posterior (synapsing at segment 7) motor neuron axons (mitochondria are more elongated), whereas in the shorter anterior axons (synapsing at segment 7) the mitochondrial morphology is comparable to controls.

(Fowler and O'Sullivan, 2016)

Expression of Rtnl1^GD900 driven by Scer\GAL4^elav-C155 (along with UAS-dcr) does not significantly affect quantal content or mini EJP amplitude (1.5 mM Ca[2+]) at the neuromuscular junction of third instar larvae compared to controls.

Expression of Rtnl1^GD900 (along with UAS-dcr) driven by Scer\GAL4^elav-C155 or Scer\GAL4^Mef2.PR or Scer\GAL4^Gli.PU (at 0.1 mM Ca[2+]) significantly reduces EJP amplitude at the third instar larval NMJ; reduction in EJP corresponds with a significant decrease in the frequency of successful synaptic transmission.

Expression of Rtnl1^GD900 (along with UAS-dcr) driven by Scer\GAL4^elav-C155 significantly increases neuromuscular junction bouton number.

(Summerville et al., 2016)

Animals expressing Rtnl1^GD900 under the control of Scer\GAL4^da.G32 show normal climbing ability as early adults, but show a progressive decline in climbing ability as they age.

Epidermal cells in third instar larvae expressing Rtnl1^GD900 under the control of Scer\GAL4^da.G32 show a striking reorganisation of the endoplasmic reticulum (ER). There is a 3-fold increase in the average length of sheet ER profiles, and denser packing of the ribosome-studded sheet ER. There is a significant increase in the ER stress response in both epidermal cells and cells of the ventral nerve cord in these animals.

Larvae expressing Rtnl1^GD900 under the control of Scer\GAL4^RapGAP1-OK6 show no defects in mitochondrial number or size in muscle 6/7 neuromuscular junction boutons in anterior abdominal segments. However, in posterior abdominal segments, the boutons of the muscle 6/7 neuromuscular junction contain fewer mitochondria than normal and they are increased in size compared to controls.

(O'Sullivan et al., 2012)

Adults expressing Rtnl1^GD900 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rtnl1^GD900, Scer\GAL4^elav-C155 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by atl^RNAi.UAS, Scer\GAL4^elav-C155

(Summerville et al., 2016)

Suppressor of

Statement

Reference

Rtnl1^GD900, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^elav-C155, atl^RNAi.UAS

(Summerville et al., 2016)

Other

Statement

Reference

ReepA⁵⁴¹, ReepB⁴⁸, Rtnl1^GD900, Scer\GAL4^tey-5053A has abnormal neuroanatomy | third instar larval stage phenotype

(Yalçın et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rtnl1^GD900, Scer\GAL4^elav-C155 has NMJ bouton | third instar larval stage phenotype, suppressible by atl^RNAi.UAS, Scer\GAL4^elav-C155

(Summerville et al., 2016)

Suppressor of

Statement

Reference

Rtnl1^GD900, Scer\GAL4^elav-C155 is a suppressor of NMJ bouton | third instar larval stage phenotype of Scer\GAL4^elav-C155, atl^RNAi.UAS

(Summerville et al., 2016)

Other

Statement

Reference

ReepA⁵⁴¹, ReepB⁴⁸, Rtnl1^GD900, Scer\GAL4^tey-5053A has axon | third instar larval stage phenotype

(Yalçın et al., 2017)

ReepA⁵⁴¹, ReepB⁴⁸, Rtnl1^GD900, Scer\GAL4^tey-5053A has endoplasmic reticulum | third instar larval stage phenotype

(Yalçın et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^tey-5053A>Rtnl1^GD900,ReepB⁴⁸;ReepA⁵⁴¹ third instar larvae frequently display gaps in the axonal endoplasmic reticulum (ER), the prevalence of the ER gaps is comparable to that observed in Rtnl1^1.W,ReepB⁴⁸;ReepA⁵⁴¹ triple mutants.

(Yalçın et al., 2017)

Co-expression of atl^{dsRNA.Scer\UAS} significantly suppresses the increases in neuromuscular junction bouton number seen in Scer\GAL4^elav-C155>Rtnl1^GD900 third instar larvae (and vice versa).

(Summerville et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v7866

w¹¹¹⁸; P{GD900}v7866

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rtnl1^GD900

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close