FlyBase Allele Report: Dmel\uif[GD139]

General Information

Symbol

Dmel\uif^GD139

Species

D. melanogaster

Name

FlyBase ID

FBal0208457

Feature type

allele

Associated gene

Dmel\uif

Associated Insertion(s)

Carried in Construct

P{GD139}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD139}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD139

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior | larval stage, with Scer\GAL4^btl.PS

(Zhang and Ward, 2009)

abnormal flight, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

decreased body size | larval stage, with Scer\GAL4^btl.PS

(Zhang and Ward, 2009)

lethal | embryonic stage, with Scer\GAL4^da.G32

(Zhang and Ward, 2009)

lethal | larval stage, with Scer\GAL4^da.G32

(Zhang and Ward, 2009)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die before end of larval stage, with Scer\GAL4^btl.PS

(Zhang and Ward, 2009)

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during larval stage, with Scer\GAL4^btl.PS

(Zhang and Ward, 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

larval tracheal system, with Scer\GAL4^btl.PS

(Zhang and Ward, 2009)

larval tracheal system, with Scer\GAL4^da.G32

(Zhang and Ward, 2009)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression via Scer\GAL4^da.G32 has no effect on development when flies are reared in normoxia or in 5% oxygen (hypoxic) conditions, compared to controls.

(Jha et al., 2016)

Adults expressing uif^GD139 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in a weak flyer phenotype.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression of uif^GD139 under the control of Scer\GAL4^btl.PS results in 5% embryonic lethality and 87% larval lethality. The mutant larvae show a growth defect.

Larvae expressing uif^GD139 under the control of Scer\GAL4^btl.PS spend very little time in the food and instead wander around the plate, even if they appear to have mostly inflated trachea (a behaviour that has been shown to be a response to hypoxia).

Expression of uif^GD139 under the control of Scer\GAL4^da.G32 results in 18% embryonic lethality and 86% larval lethality.

Expression of uif^GD139 under the control of either Scer\GAL4^btl.PS or Scer\GAL4^da.G32 results in a nearly completely penetrant tracheal defect in newly hatched larvae. The expressivity of the phenotype is variable.

Expression of uif^GD139 under the control of Scer\GAL4^en-e16E results in almost no embryonic lethality and animals have completely inflated tracheae.

(Zhang and Ward, 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v1051

w¹¹¹⁸; P{GD139}v1051

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

SP1070^GD139

uif^GD139

Name Synonyms

P{GD139}

(Lassetter et al., 2023)

Secondary FlyBase IDs

References (10)

Report Sections

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