UASt regulatory sequences drive expression of an inverted repeat.
Scer\GAL4tub.PU- or Scer\GAL4elav.PU-, but not Scer\GAL4repo.PU-mediated expression of Ten-aGD3330 destroys the morphology of the ellipsoid body and fan-shaped body within the central complex of the brain.
Adults expressing Ten-aGD3330 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in a weak flyer phenotype.