FlyBase Allele Report: Dmel\Vha100-1[GD12710]

General Information

Symbol

Dmel\Vha100-1^GD12710

Species

D. melanogaster

Name

FlyBase ID

FBal0208960

Feature type

allele

Associated gene

Dmel\Vha100-1

Associated Insertion(s)

Carried in Construct

P{GD12710}

Also Known As

UAS-Vha100-1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12710}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12710

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Baisgaard et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Hsap\MAPT^UAS.cWa

(Williamson and Hiesinger, 2010)

modeled by Hsap\MAPT^R406W.UAS

(Williamson and Hiesinger, 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal phototaxis | adult stage, with Scer\GAL4^GMR.PF

(Dubos et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

eye photoreceptor cell | adult stage, with Scer\GAL4^GMR.PF

(Dubos et al., 2015)

pigment cell | adult stage, with Scer\GAL4^GMR.PF

(Dubos et al., 2015)

Detailed Description

Statement

Reference

Flies with expression of Vha100-1^GD12710 driven by Scer\GAL4^GMR.PF (together with UAS-dicer2 to enhance RNAi efficiency) show significant defects in phototaxis behavior compared to controls. Mutant ommatidia and photoreceptors show overall intact organization but photoreceptor-surrounding pigment cells are highly vacuolated and there is an accumulation of vesicular structures in photoreceptor cytoplasm.

(Dubos et al., 2015)

Adults expressing Vha100-1^GD12710 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression of Vha100-1^GD12710 under the control of Scer\GAL4^GMR.PF does not result in significant photoreceptor degeneration after two days intense light stimulation, as measured by ERG response amplitudes.

(Williamson and Hiesinger, 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12710

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Vha100-1^GD12710, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Williamson and Hiesinger, 2010)

Vha100-1^GD12710, Scer\GAL4^GMR.PF is an enhancer of abnormal neurophysiology phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Williamson and Hiesinger, 2010)

Vha100-1^GD12710, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Williamson and Hiesinger, 2010)

Vha100-1^GD12710, Scer\GAL4^GMR.PF is an enhancer of abnormal neurophysiology phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Williamson and Hiesinger, 2010)

Other

Statement

Reference

Hsap\APP^{Aβ40.UAS.cUa}, Scer\GAL4^GMR.PF, Vha100-1^GD12710 has abnormal neurophysiology phenotype

(Williamson and Hiesinger, 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Vha100-1^GD12710, Scer\GAL4^GMR.PF is an enhancer of photoreceptor neuron phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Williamson and Hiesinger, 2010)

Vha100-1^GD12710, Scer\GAL4^GMR.PF is an enhancer of photoreceptor neuron phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Williamson and Hiesinger, 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Flies co-expressing Vha100-1^GD12710 and Hsap\APP^{Aβ40.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PF and exposed to two days intense light stimulation show a significantly reduced ERG response amplitude.

Co-expression of Hsap\MAPT^Scer\UAS.cWa with Vha100-1^GD12710 in developing eyes exposed to two days of intense light stimulation accelerates the degenerative photoreceptor phenotype.

Co-expression of Hsap\MAPT^{R406W.Scer\UAS} with Vha100-1^GD12710 in developing eyes exposed to two days of intense light stimulation accelerates the degenerative photoreceptor phenotype.

(Williamson and Hiesinger, 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v22924

w¹¹¹⁸; P{GD12710}v22924

Notes on Origin

Discoverer