FlyBase Allele Report: Dmel\Vps28[GD7696]

General Information

Symbol

Dmel\Vps28^GD7696

Species

D. melanogaster

Name

FlyBase ID

FBal0208992

Feature type

allele

Associated gene

Dmel\Vps28

Associated Insertion(s)

Carried in Construct

P{GD7696}

Also Known As

Vps28 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7696}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7696

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Vps28^GD7696 under the control of Scer\GAL4^c564 does not lead to significant changes in the average adult fat body cell size, as compared to controls.

(Neyen et al., 2016)

Adults expressing Vps28^GD7696 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7696

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Vps28^GD7696, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Alexopoulou et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

Vps28^GD7696, Scer\GAL4^GMR.PU is an enhancer of eye | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Alexopoulou et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype characteristic for adult flies expressing Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PU is worsened further by co-expression of Vps28^GD7696.

(Alexopoulou et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v31894

w¹¹¹⁸; P{GD7696}v31894

Notes on Origin

Discoverer