FlyBase Allele Report: Dmel\bru1[GD8699]

General Information

Symbol

Dmel\bru1^GD8699

Species

D. melanogaster

Name

FlyBase ID

FBal0209071

Feature type

allele

Associated gene

Dmel\bru1

Associated Insertion(s)

Carried in Construct

P{GD8699}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8699}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8699

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Shulman et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Act88F.PB

(Oas et al., 2014)

flightless, with Scer\GAL4^Act88F.PU

(Nikonova et al., 2019)

flightless, with Scer\GAL4^Mef2.PR

(Spletter et al., 2015, Schnorrer et al., 2010)

flightless, with Scer\GAL4^Mef2.PU

(Nikonova et al., 2019)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Spletter et al., 2015)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Nikonova et al., 2022, Spletter et al., 2015, Schnorrer et al., 2010)

myofibril, with Scer\GAL4^Mef2.PR

(Spletter et al., 2015, Schnorrer et al., 2010)

sarcomere, with Scer\GAL4^Mef2.PR

(Nikonova et al., 2022, Spletter et al., 2015, Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Flies expressing aret^GD8699 under the control of Scer\GAL4^Mef2.PR are viable but entirely flightless. The indirect flight muscle fibers of day 1 adults begin to thin and rupture close to their thoracic attachment sites. Additionally, the sarcomeres appear too short and are sometimes lost. Myofibrils are variable in diameter and often hollow, in contrast to dense, regular myofibrils in wild type. A few days after eclosion, generally all IFM fibers are ruptured and the myofibrils entirely lose their sarcomeric organisation. The sarcomeres are of comparable length to wild type at 48 hours after puparium formation (APF), but fail to grow and even shorten by 72 hours APF. The tubular leg muscles do not display any obvious phenotypes.

(Spletter et al., 2015)

Flies expressing aret^GD8699 under the control of Scer\GAL4^Act88F.PB have significant defects in flight ability compared to wild type.

(Oas et al., 2014)

Adults expressing aret^GD8699 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in missing adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in degenerate myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in missing sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8699

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Mef2.PR, bru1^GD8699 has indirect flight muscle cell phenotype, suppressible by Mhc¹⁰

(Spletter et al., 2015)

Other

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR, bru1^GD8699 has indirect flight muscle cell phenotype

(Nikonova et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Mhc¹⁰ completely suppresses the indirect flight muscle degeneration seen in newly eclosed flies expressing aret^GD8699 under the control of Scer\GAL4^Mef2.PR.

(Spletter et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v41568

w¹¹¹⁸; P{GD8699}v41568

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8699

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

aret^GD8699

bru1^GD8699

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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