Expressing Cul4^GD14006 under the control of Scer\GAL4^insc-Mz1407 leads to larval brain neuroblasts showing signs of quiescence: neuroblasts show a significant decrease in cell divisions (both DNA replication/EdU staining and Mitosis/phospho-H3); and frequently show a primary cellular process, whereas control neuroblasts show a typically rounded morphology. (Experiments were done in combination with Dicer-2 for higher RNAi efficiency).

Expression of Cul4^GD14006 under the control of Scer\GAL4^Cg.PA leads to lethality during early larval stages.

Expression of Cul4^GD14006 in somatic cyst cells under the control of any of Scer\GAL4^tj-NP1624, Scer\GAL4^A3.eya or Scer\GAL4^c784 (limited to a week from eclosion onwards using Scer\GAL80^ts.αTub84B) does not disrupt germline enclosure in testes, and cytoplasmic extensions from the cyst cells are clearly seen in the expected net-like pattern around the germline.

Adults expressing Cul4^GD14006 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^Mef2.PR results in late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in rounded larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with irregular aggregates of Z-lines in the larval body wall muscles.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum.