FlyBase Allele Report: Dmel\Desat1[GD2950]

General Information

Symbol

Dmel\Desat1^GD2950

Species

D. melanogaster

Name

FlyBase ID

FBal0209343

Feature type

allele

Associated gene

Dmel\Desat1

Associated Insertion(s)

Carried in Construct

P{GD2950}

Also Known As

UAS-Desat1 RNAi, UAS-desat1-RNAi, UAS-desat1-IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2950}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2950

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of restrictive cardiomyopathy

CEA

(Tuthill Ii et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior, with Scer\GAL4^Desat1.-1102

(Bousquet et al., 2012)

abnormal starvation stress response, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

abnormal stress response | larval stage, with Df(3R)Exel7316, Scer\GAL4^ppl.PP

(Köhler et al., 2009)

decreased body size, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

decreased body weight | adult stage, with Scer\GAL4^r4

(Tuthill Ii et al., 2021)

decreased body weight | larval stage, with Scer\GAL4^r4

(Tuthill Ii et al., 2021)

decreased cell size, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

decreased rate of feeding behavior, with Scer\GAL4^r4

(Tuthill Ii et al., 2021)

decreased size | adult stage, with Scer\GAL4^r4

(Tuthill Ii et al., 2021)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult gut, with Scer\GAL4^r4

(Tuthill Ii et al., 2021)

adult heart, with Scer\GAL4^r4

(Tuthill Ii et al., 2021)

adult heart | nutrition conditional, with Scer\GAL4^r4

(Tuthill Ii et al., 2021)

wing, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

Detailed Description

Statement

Reference

Adults expressing Desat1^GD2950 under the control of Scer\GAL4^r4 exhibit decreased food consumption and increased excreta; guts are smaller (decreased gut length and width) and less permeable (fewer Smurfs in Smurf assays).

Under a control diet, 3-weeks old adult females expressing Desat1^GD2950 under the control of Scer\GAL4^r4 show decreased larval and fly body mass, decreased heart failure, with faster recovery, as compared to controls. Under high sugar diet, these individuals show increased heart failure, with slower recovery, decreased heart rate, increased heart period, decreased systolic and diastolic width, and increased systolic interval.

(Tuthill Ii et al., 2021)

Scer\GAL4^Cg.PA-mediated expression of Desat1^GD2950 results in smaller pupae and adults with reduced wing/body size. The larval fat body is composed of smaller cells.

The half-life of Scer\GAL4^Cg.PA Desat1^GD2950 animals during starvation is decreased compared to controls.

(Parisi et al., 2013)

Expression of desat1^GD2950 under the control of Scer\GAL4^desat1.RE in males results in the ratio of desaturated:saturated linear hydrocarbons being biased in favour saturated linear hydrocarbons, in contrast to wild-type males where the ratio is biased in favour of desaturated hydrocarbons.

The ratio of desaturated:saturated linear hydrocarbons is reduced in males expressing desat1^GD2950 under the control of either Scer\GAL4^desat1.RA or Scer\GAL4^desat1.RC, and to a lesser extent Scer\GAL4^desat1.RB, compared to the ratio seen in wild-type males.

The ratio of desaturated:saturated linear hydrocarbons is reduced in males expressing desat1^GD2950 under the control of either Scer\GAL4^desat1.RD or Scer\GAL4^desat1.-1102 is not significantly altered compared to the ratio seen in wild-type males.

The ability of males expressing desat1^GD2950 under the control of any one of Scer\GAL4^desat1.RA, Scer\GAL4^desat1.RC, Scer\GAL4^desat1.RE, Scer\GAL4^desat1.RB or Scer\GAL4^desat1.RD to discriminate between male and female flies in a courtship assay is not significantly altered compared to wild type.

Males expressing desat1^GD2950 under the control of Scer\GAL4^desat1.-1102 cannot discriminate between male and female flies in a courtship assay.

(Bousquet et al., 2012)

Adults expressing Desat1^GD2950 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Larvae expressing desat1^GD2950 under the control of Scer\GAL4^ppl.PP in a heterozygous Df(3R)Exel7316 genetic background show abnormal autophagic response to starvation.

(Köhler et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2950

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Desat1^GD2950, Scer\GAL4^Cg.PA is a suppressor of increased body size | pupal stage phenotype of Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

Desat1^GD2950, Scer\GAL4^Cg.PA is a suppressor of abnormal starvation stress response phenotype of Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Desat1^GD2950, Scer\GAL4^Cg.PA is a suppressor of wing phenotype of Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Desat1^GD2950 prevents the Scer\GAL4^Cg.PA-mediated expression of Myc^{Scer\UAS.T:Ivir\HA1} resulting in abnormally large pupae and large wings. Co-expression of Desat1^GD2950 also prevents the increased half-life upon starvation seen in Scer\GAL4^Cg.PA Myc^{Scer\UAS.T:Ivir\HA1} animals.

(Parisi et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v33338

w¹¹¹⁸; P{GD2950}v33338

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2950

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Desat1^GD2950

desat1^GD2950

Name Synonyms

Secondary FlyBase IDs

References (12)

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