FlyBase Allele Report: Dmel\eIF4A[GD14111]

General Information

Symbol

Dmel\eIF4A^GD14111

Species

D. melanogaster

Name

FlyBase ID

FBal0209465

Feature type

allele

Associated gene

Dmel\eIF4A

Associated Insertion(s)

Carried in Construct

P{GD14111}

Also Known As

UAS-eIF4A RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14111}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14111

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cyst progenitor cell | decreased number, with Scer\GAL4^tj.PU

(Wang et al., 2025)

embryonic/larval optic stalk | third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

eye disc | third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

glial cell | third instar larval stage, with Scer\GAL4^repo

(Tavares et al., 2015)

Detailed Description

Statement

Reference

Expressing eIF4A^GD14111 under the control of Scer\GAL4^repo results in decreased glial cell numbers in the eye discs and optic stalks of third instar larvae. Photoreceptor axon targeting to the optic stalk is unaffected. GAL80ts was used to induce eIF4A^GD14111 expression from 72 hours after larval hatching onwards.

(Tavares et al., 2015)

Adults expressing eIF-4a^GD14111 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early larval lethality or late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality (either before or at the pupal stage), depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development