FlyBase Allele Report: Dmel\en[GD13214]

General Information

Symbol

Dmel\en^GD13214

Species

D. melanogaster

Name

FlyBase ID

FBal0209515

Feature type

allele

Associated gene

Dmel\en

Associated Insertion(s)

Carried in Construct

P{GD13214}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD13214}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD13214

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

embryonic leading edge cell, with Scer\GAL4^ptc-559.1

(Gettings et al., 2010)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Expression of en^GD13214 under the control of Scer\GAL4^ptc-559.1 results in reduced cell mixing at the segment boundary during dorsal closure in the embryo, with 40% of segments showing partial or no mixing (in wild-type embryos, a "mixer cell" moves across the segment boundary from the anterior compartment to the posterior compartment at the end of dorsal closure).

(Gettings et al., 2010)

Adults expressing en^GD13214 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing en^GD13214 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in defects in wing posture.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in mild thorax closure defects.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13214

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR42B09, en^GD13214, inv^HMS02209 has decreased fecundity | female phenotype, enhanceable by Ecol\lacZ[+]/Ecol\lacZ^bab-P

(Allbee et al., 2018)

Other

Statement

Reference

Scer\GAL4^GMR42B09, en^GD13214, inv^HMS02209 has decreased fecundity | female phenotype

(Allbee et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR42B09, en^GD13214, inv^HMS02209 has ovary | adult stage phenotype, enhanceable by Ecol\lacZ[+]/Ecol\lacZ^bab-P

(Allbee et al., 2018)

Other

Statement

Reference

Scer\GAL4^GMR42B09, en^GD13214, inv^HMS02209 has ovary | adult stage phenotype

(Allbee et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing en^GD13214 and inv^HMS02209 under the control of Scer\GAL4^GMR42B09 induces mild-to-strong ovary defects and females lay significantly fewer eggs than controls.

(Allbee et al., 2018)

Xenogenetic Interactions

Statement

Reference

The mild-to-strong ovary defects and the decreased egg laying induced by the co-expression of en^GD13214 and inv^HMS02209 under the control of Scer\GAL4^GMR42B09 are enhanced by one copy of Ecol\lacZ^bab-P.

(Allbee et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v35697

w¹¹¹⁸; P{GD13214}v35697

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13214

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Engrailed^RNAi

(Allbee et al., 2018)

en^GD13214

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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