FlyBase Allele Report: Dmel\hh[GD193]

General Information

Symbol

Dmel\hh^GD193

Species

D. melanogaster

Name

FlyBase ID

FBal0209688

Feature type

allele

Associated gene

Dmel\hh

Associated Insertion(s)

Carried in Construct

P{GD193}

Also Known As

UAS-hh^RNAi, Hh RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD193}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD193

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

increased cell number | larval stage, with Scer\GAL4^NP1029

(Destalminil-Letourneau et al., 2021)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic/larval crystal cell | increased number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

embryonic/larval crystal cell | increased number | larval stage, with Scer\GAL4^NP1029

(Destalminil-Letourneau et al., 2021)

Detailed Description

Statement

Reference

Expression of hh^GD193 does not significantly affect wing patterning (driven by Scer\GAL4^nub-AC-62) or thermal allodynia (driven by Scer\GAL4^ppk.1.9, even with co-expression of hh^NIG.4637R).

(Im et al., 2015)

Expressing hh^GD193 under the control of Scer\GAL4^ey.PH does not cause any overt eye overgrowth.

(Da Ros et al., 2013)

Adults expressing hh^GD193 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of hh^GD193 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD193} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

hh^GD193, Scer\GAL4^ey.PH is an enhancer of visible | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Da Ros et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

hh^GD193, Scer\GAL4^ey.PH is an enhancer of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Da Ros et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

hh^GD193 enhances the eye overgrowth phenotype seen when Dl^Scer\UAS.cDa is expressed under the control of Scer\GAL4^ey.PH. 30% of flies exhibit eye overgrowth.

(Da Ros et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v1402

w¹¹¹⁸; P{GD193}v1402

v1403

w¹¹¹⁸; P{GD193}v1403

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

hh^GD193

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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