FlyBase Allele Report: Dmel\key[GD1249]

General Information

Symbol

Dmel\key^GD1249

Species

D. melanogaster

Name

FlyBase ID

FBal0209782

Feature type

allele

Associated gene

Dmel\key

Associated Insertion(s)

Carried in Construct

P{GD1249}

Also Known As

UAS-Key RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1249}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1249

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates adult T-cell leukemia/lymphoma

modeled by HTLV-1\Tax^UAS.Tag:MYC

(Shirinian et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | adult stage, with Scer\GAL4^Cg.PA

(Molaei et al., 2019)

hyperplasia, with Scer\GAL4^mex1.2.1

(Chen et al., 2014)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult fat body | nutrition conditional, with Scer\GAL4^Cg.PA

(Molaei et al., 2019)

adult midgut, with Scer\GAL4^mex1.2.1

(Chen et al., 2014)

Detailed Description

Statement

Reference

Expressing key^GD1249 under the control of Scer\GAL4^Cg.PA results in a significantly reduced resistance to starvation and decreased lipid storage in the fat body of starved adult flies.

(Molaei et al., 2019)

Expression of key^GD1249 under the control of Scer\GAL4^mex1.2.1 results in increased midgut hyperplasia in 5 day old flies compared to controls.

The age-associated hyperplasia seen in 50 day old control flies is reduced when key^GD1249 is expressed in the fat body under the control of Scer\GAL4^Cg.PA.

(Chen et al., 2014)

Adults expressing key^GD1249 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development