FlyBase Allele Report: Dmel\krz[GD8470]

General Information

Symbol

Dmel\krz^GD8470

Species

D. melanogaster

Name

FlyBase ID

FBal0209800

Feature type

allele

Associated gene

Dmel\krz

Associated Insertion(s)

Carried in Construct

P{GD8470}

Also Known As

krz RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8470}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8470

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^Bx-MS1096

(Chai et al., 2019)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

melanotic mass phenotype | third instar larval stage, with Scer\GAL4^Cg.PA

(Anjum et al., 2013)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Chai et al., 2019)

Phenotype Manifest In

lamellocyte | increased number | third instar larval stage, with Scer\GAL4^Cg.PA

(Anjum et al., 2013)

melanotic mass | third instar larval stage, with Scer\GAL4^Cg.PA

(Anjum et al., 2013)

wing blade, with Scer\GAL4^Bx-MS1096

(Chai et al., 2019)

Detailed Description

Statement

Reference

The expression of krz^GD8470 under the control of Scer\GAL4^Bx-MS1096 (in combination with Dicer-2, for a more efficient RNAi) leads to unexpanded and severely wrinkled wings.

(Chai et al., 2019)

Third instar larvae expressing krz^GD8470 via Scer\GAL4^Cg.PA, in the presence of P{UAS-Dcr-2.D}, exhibit melanotic masses and a 3-fold increase in circulating lamellocytes. When expressed via Scer\GAL4^Cg.PA and in the absence of P{UAS-Dcr-2.D}, no melanotic masses or increase in lamellocytes are observed.

When expressed via Scer\GAL4^da.G32 in the absence of P{UAS-Dcr-2.D}, animals survive to adulthood.

(Anjum et al., 2013)

Adults expressing krz^GD8470 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8470

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, krz^GD8470 has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^Bx-MS1096/mthl1^GD727

(Chai et al., 2019)

Scer\GAL4^Bx-MS1096, krz^GD8470 has abnormal size | adult stage phenotype, suppressible | partially by Scer\GAL4^Bx-MS1096/mthl1^GD727

(Chai et al., 2019)

Other

Statement

Reference

Scer\GAL4^Cg.PA, Ulp1^GD7581, krz^GD8470 has melanotic mass phenotype | third instar larval stage phenotype

(Anjum et al., 2013)

Scer\GAL4^da.G32, Ulp1^GD7581, krz^GD8470 has lethal - all die before end of larval stage phenotype

(Anjum et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, krz^GD8470 has wing blade phenotype, suppressible | partially by Scer\GAL4^Bx-MS1096/mthl1^GD727

(Chai et al., 2019)

Enhancer of

Statement

Reference

krz^GD8470, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Enhancer of

Statement

Reference

krz^GD8470, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Suppressor of

Statement

Reference

krz^GD8470, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

Other

Statement

Reference

Scer\GAL4^Cg.PA, Ulp1^GD7581, krz^GD8470 has melanotic mass | third instar larval stage phenotype

(Anjum et al., 2013)

Scer\GAL4^Cg.PA, Ulp1^GD7581, krz^GD8470 has lamellocyte | increased number | third instar larval stage phenotype

(Anjum et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Whereas Scer\GAL4^Cg.PA-mediated expression of Ulp1^GD7581 or krz^GD8470, in the absence of P{UAS-Dcr-2.D}, does not result in a melanotic mass or circulating lamellocyte phenotype, their co-expression results in the formation of multiple melanotic masses and 10-fold higher levels of circulating lamellocytes.

Whereas Scer\GAL4^da.G32-mediated expression of Ulp1^GD7581 or krz^GD8470, in the absence of P{UAS-Dcr-2.D}, does not result in lethality, their co-expression results in 100% at larval stages.

(Anjum et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v41559

w¹¹¹⁸; P{GD8470}v41559

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8470

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

krz^GD8470

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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