FlyBase Allele Report: Dmel\lola[GD4071]

General Information

Symbol

Dmel\lola^GD4071

Species

D. melanogaster

Name

FlyBase ID

FBal0209938

Feature type

allele

Associated gene

Dmel\lola

Associated Insertion(s)

Carried in Construct

P{GD4071}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4071}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4071

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

abdominal dorsal multidendritic neuron ddaD | third instar larval stage, with Scer\GAL4²²¹

(Hattori et al., 2013)

abdominal dorsal multidendritic neuron ddaE | third instar larval stage, with Scer\GAL4²²¹

(Hattori et al., 2013)

dendritic tree | third instar larval stage, with Scer\GAL4²²¹

(Hattori et al., 2013)

dendritic tree | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

embryonic/larval lymph gland, with Scer\GAL4^HCH.Hand, Scer\GAL4^Hml.Δ

(Mondal et al., 2014)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011, Neumüller et al., 2011)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

type II neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

Detailed Description

Statement

Reference

Expression via "HHLT-gal4" results in loss of progenitor cells in the primary lobes of the larval lymph gland. ("HHLT-gal4" is a combination of Scer\GAL4^HCH.Hand and Scer\GAL4^Hml.Δ that express in early lymph gland blood cells (hemocytes) and circulating and sessile blood cells, respectively, and incorporates Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} to maintain expression in the lymph gland after Scer\GAL4^HCH.Hand is down-regulated.)

(Mondal et al., 2014)

The expression of lola^GD4071 under the control of Scer\GAL4²²¹, in combination with Dicer-2 for efficient RNAi, results in third instar larval class I abdominal dorsal multidendritic neurons ddaD and ddaE exhibiting a significant decrease in the number of dendrite branch terminals, as compared to controls.

Expressing lola^GD4071 under the control of Scer\GAL4^ppk.PG, in combination with Dicer-2 for efficient RNAi, results in third intar larval class IV abdominal dorsal multidendritic neurons ddaC exhibiting dendrite branch terminals of similar number but of significantly lower average length, as compared to controls.

(Hattori et al., 2013)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increase in the number of neuroblasts in the larval brain compared to controls.

(Neumüller et al., 2011)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in overproliferation of type II neuroblast lineages.

(Neumüller et al., 2011)

Adults expressing lola^GD4071 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in a weak flyer phenotype.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 10-20% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in strong thorax closure defects.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development