FlyBase Allele Report: Dmel\mbt[GD9608]

General Information

Symbol

Dmel\mbt^GD9608

Species

D. melanogaster

Name

FlyBase ID

FBal0210055

Feature type

allele

Associated gene

Dmel\mbt

Associated Insertion(s)

Carried in Construct

P{GD9608}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9608}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9608

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

RNAi knockdown of mbt, through expression of mbt^GD9608 in the posterior compartment of the wing disc, under the control of either Scer\GAL4^en.PU or Scer\GAL4^hh.PU, does not affect levels of cell death.

(Wong et al., 2014)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 30-40% or 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9608

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, mad2^GD16427, mbt^GD9608 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, mbt^GD9608 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Other

Statement

Reference

Scer\GAL4^hh.PU, mad2^GD16427, mbt^GD9608 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, mbt^GD9608 has increased cell death phenotype

(Wong et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, mad2^GD16427, mbt^GD9608 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, mbt^GD9608 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Other

Statement

Reference

Scer\GAL4^hh.PU, mad2^GD16427, mbt^GD9608 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, mbt^GD9608 has wing disc phenotype

(Wong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when mbt^GD9608 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant increase in cell death in these double mutants.

(Wong et al., 2014)

Xenogenetic Interactions

Statement

Reference

(Wong et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v46044

w¹¹¹⁸; P{GD9608}v46044

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9608

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mbt^GD9608

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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