FlyBase Allele Report: Dmel\mod(mdg4)[GD16547]

General Information

Symbol

Dmel\mod(mdg4)^GD16547

Species

D. melanogaster

Name

FlyBase ID

FBal0210093

Feature type

allele

Associated gene

Dmel\mod(mdg4)

Associated Insertion(s)

Carried in Construct

P{GD16547}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD16547}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD16547

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Zzzz\CTG^{86.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

modeled by Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

modeled by Zzzz\CAG^{93.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

wing, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

Detailed Description

Statement

Reference

The expression of mod(mdg4)^GD16547 under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) leads to small and folded wings, as compared to controls.

(Hevia et al., 2017)

Adults expressing mod(mdg4)^GD16547 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Scer\GAL4^GMR.PFa, Zzzz\CTG^{86.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^nub.PU/mod(mdg4)^GD16547 is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa is a suppressor | partially of viable phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

Other

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\CTG^{term.CTG.99.UAS}, mod(mdg4)^GD16547 has visible phenotype

(van Eyk et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{93.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\CTG^{86.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^nub.PU/mod(mdg4)^GD16547 is a suppressor of wing phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

Other

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\CTG^{term.CTG.99.UAS}, mod(mdg4)^GD16547 has eye phenotype

(van Eyk et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of mod(mdg4)^GD16547 (together with Dicer-2, for efficient RNAi) suppresses the oversized wing defects induced by the expression of Smox^{SDVD.Scer\UAS} under the control of Scer\GAL4^nub.PU.

(Hevia et al., 2017)

Xenogenetic Interactions

Statement

Reference

Co-expression of mod(mdg4)^GD16547 with Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa results in an enhancement of the Zzzz\CAG^{93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}-induced eye phenotype and a reduction in viability of the flies.

Co-expression of mod(mdg4)^GD16547 with Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa results in a mild enhancement of the Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}-induced eye pigmentation defects.

Co-expression of mod(mdg4)^GD16547 with Zzzz\CTG^{86.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa results in the enhancement of the Zzzz\CTG^{86.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}-induced eye phenotype.

Co-expression of mod(mdg4)^GD16547 with Zzzz\CTG^{term.CTG.99.Scer\UAS} under the control of Scer\GAL4^GMR.PFa results in a marked disruption of the pigmentation and patterning of the eye.

Co-expression of mod(mdg4)^GD16547 with Zzzz\CAG^{term93.Scer\UAS} under the control of Scer\GAL4^GMR.PFa does not disrupt eye patterning.

Co-expression of mod(mdg4)^GD16547 with Zzzz\CAG^{term.CAA.114.Scer\UAS} under the control of Scer\GAL4^GMR.PFa does not disrupt eye patterning.

Co-expression of mod(mdg4)^GD16547 with Hsap\ATXN10^{rAUUCU.65.Scer\UAS} under the control of Scer\GAL4^GMR.PFa does not disrupt eye patterning.

(van Eyk et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v52268

w¹¹¹⁸ P{GD16547}v52268

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mod(mdg4)^GD16547

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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