Expression of olf186-F^GD3554 under the control of Scer\GAL4^elav-C155 leads to significant decrease in flight ability in adults (measured as flight time in a single flight assay on tethered flies).

Expression of olf186-F^GD3554 leads to third instar larvae exhibiting a significant decrease in the number of dendrite branch terminals in either class I ddaD and ddaE (when expression is driven by Scer\GAL4²²¹, in combination with Dicer-2 for efficient RNAi) or class IV ddaC (when expression is driven by Scer\GAL4^ppk.PG, in combination with Dicer-2 for efficient RNAi) abdominal dorsal multidendritic neurons, as compared to controls.

Flies expressing olf186-F^GD3554 under the control of Scer\GAL4^Trh.PB show normal flight ability in a cylinder drop test. However, the flies show increased spontaneous firing from the dorsal longitudinal indirect flight muscles compared to wild type.

Expression under the control of Scer\GAL4^Mef2.PR results in early larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

No obvious changes are observed in olf186-F^GD3554 larvae. They are viable and pupate normally. Adult flies with pan-neural expression of olf186-F^GD3554 (under the control of Scer\GAL4^elav-C155) exhibit defective wing posture with significant loss of flight, as seen in the 'cylinder drop' test assay. Over 50% of flies with olf186-F^GD3554 knockdown (under the control of Scer\GAL4^elav-C155) are flightless. Expression of olf186-F^GD3554 in glutamatergic neurons (under the control of Scer\GAL4^OK371), which include the flight motor neurons, reduces flight ability in ~35% of adult Drosophila. Nonfliers with pan-neural expression of olf186-F^GD3554 selected from the cylinder drop test are either unable to initiate rhythmic action potentials in response to an air-puff stimulus or exhibit unsustained (<5s) and arrhythmic flight patters. Knockdown of olf186-F^GD3554 in glutamatergic neurons leads to a milder change in flight patterns compared with pan-neural knockdown. Recordings from resting DLMs of these flies reveals a high arrhythmic spontaneous firing rate of action potentials, the frequency of which is significantly higher than wild-type.

Orai^GD3554, Scer\GAL4²²¹ is a suppressor of abdominal dorsal multidendritic neuron ddaD | third instar larval stage phenotype of Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

Orai^GD3554, Scer\GAL4²²¹ is a suppressor of abdominal dorsal multidendritic neuron ddaE | third instar larval stage phenotype of Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

Orai^GD3554, Scer\GAL4²²¹ is a suppressor of dendritic tree | third instar larval stage phenotype of Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}