FlyBase Allele Report: Dmel\poe[GD8339]

General Information

Symbol

Dmel\poe^GD8339

Species

D. melanogaster

Name

FlyBase ID

FBal0210371

Feature type

allele

Associated gene

Dmel\poe

Associated Insertion(s)

Carried in Construct

P{GD8339}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8339}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8339

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adults expressing poe^GD8339 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 80-90% or 90-100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8339

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^salm.EPv/poe^GD8339 is an enhancer of visible | adult stage phenotype of Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS

(Ashton-Beaucage et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^salm.EPv/poe^GD8339 is an enhancer of wing blade phenotype of Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv/poe^GD8339 is an enhancer of wing vein phenotype of Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS

(Ashton-Beaucage et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wein material in the adult wing characteristic for flies expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^salm.EPv is partially suppressed by co-expression of either Usp47^GD10642 or Usp47^NIG.5486R. The ameliorated phenotype of Scer\GAL4^salm.EPv,rl^{Sem.S.Scer\UAS};Usp47^GD10642 flies can be worsened again by co-expression of either poe^GD8339 or poe^KK101471 : the severity of their ectopic venation phenotype is comparable to that of flies expressing rl^{Sem.S.Scer\UAS} only.

(Ashton-Beaucage et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v17648

w¹¹¹⁸; P{GD8339}v17648

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8339

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

poe^GD8339

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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